PILOT STUDY--INDUCTION OF VIRAL ACUTE HEPATITIS BY HEPATITIS B VIRUS GENES

中试研究--乙型肝炎病毒基因诱导病毒性急性肝炎

• 批准号: 3754250
• 负责人: ALEEM SIDDIQUI
• 金额: --
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3754250
• 关键词: Hepatovirus; biomedical facility; genetic regulation; hepatitis B; liver; molecular pathology; virus DNA; virus RNA; virus genetics

Human hepatitis B virus (HBV) belongs to a family of hepadnaviruses which replicate via the reverse transcription of an RNA intermediate, the pregenome. HBV infection represents a major worldwide human health problem. It causes acute, chronic hepatitis and the infection has been linked with hepatocellular carcinoma (HCC) in humans. An understanding of the biology of HBV has been severely limited due to its narrow host range and the inability to propagate in in vitro. The earliest consequence of HBV infection is the occurrence of acute phase of viral hepatitis B. This results in elevated levels of liver enzymes. In this study, our basic inquiry relates to whether HBV X gene product is responsible for the transcriptional activation of acute phase genes of the liver. Studies are proposed here to investigate in detail the mechanism of acute viral hepatitis resulting from HBV infections. In this pilot feasibility project we propose to study the acute-phase of viral hepatitis by investigating the role HBV genes, with particular emphasis on X gene product in the transcriptional activation of acute-phase genes in the liver.

人乙肝病毒(HBVs)属于一种肝病毒家族， 通过RNA中间体的逆转录进行复制 前基因组。乙肝病毒感染是世界范围内人类健康的主要问题 有问题。它会导致急性、慢性肝炎，感染已经 与人类的肝细胞癌(HCC)有关。一种对 由于其宿主范围狭窄，乙肝病毒的生物学特性受到严重限制。 以及无法在体外繁殖。最早的后果是 乙肝病毒感染是发生在乙型病毒性肝炎的急性期。 会导致肝酶水平升高。在这项研究中，我们的基本 询问是否与HBVX基因产物有关 肝脏急性期基因的转录激活。研究是 建议在此详细研究急性病毒感染的发病机制。 由乙肝病毒感染引起的肝炎。 在这项试验可行性计划中，我们建议研究急性期 病毒性肝炎通过研究乙肝病毒基因的作用，特别是 重视X基因产物在急性期转录激活中的作用 肝脏中的基因。