CHARACTERIZATION OF TST, THE EIGHTH MEMBER OF THE FGF GENE FAMILY

FGF 基因家族第八个成员 TST 的特征

• 批准号: 3752787
• 负责人: T MIKI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752787
• 关键词: 3T3 cells; DNA replication; RNase protection assay; animal genetic material tag; cell transformation; complementary DNA; epithelium; fibroblast growth factor; fibroblasts; genetic library; gestational age; glia; human genetic material tag; human tissue; mitogens; molecular cloning; structural genes; testis; transfection

The ectopic expression of cDNAs in mammalian cells has been widely used to isolate genes encoding growth factors and their receptors. Transfection of a mouse testis cDNA library into NIH/3T3 fibroblasts resulted in the isolation of several cDNA clones with high titer transforming capacity. The sequence of one of these clones. Tst. a 1.6 kB cDNA with an open reading frame encoding a protein of 208 amino acids. revealed that Tst was the eighth member of the fibroblast growth factor (FGF) gene family. Conditioned medium from NIH/3T3 cells expressing Tst stimulated DNA synthesis in fibroblasts. epithelial cells and glial cells. indicating a broad spectrum of mitogenic activity. The Tst cDNA detected a 1.6 kB mRNA in RNA isolated from adult mouse testis. but not in RNA isolated from mouse liver. brain. heart or kidney. Ribonuclease protection analysis demonstrated that the abundance of Tst mRNA was highest during embryonic day 11 and 13 of mouse embryonic development. In addition. a partial human FGF-8 cDNA was isolated by screening a human testis cDNA library with the Tst cDNA.

哺乳动物细胞中cDNA的异位表达已被广泛应用 分离编码生长因子及其受体的基因。 将小鼠睾丸 cDNA 文库转染至 NIH/3T3 成纤维细胞 结果分离出几个具有高滴度的 cDNA 克隆 转化能力。 这些克隆之一的序列。测试。 1.6 kB cDNA，具有编码 208 个氨基酸的蛋白质的开放阅读框。 揭示Tst是成纤维细胞生长因子的第八个成员 (FGF) 基因家族。 来自表达 Tst 的 NIH/3T3 细胞的条件培养基 刺激成纤维细胞中的 DNA 合成。上皮细胞和神经胶质细胞 细胞。表明具有广谱的有丝分裂活性。 Tst cDNA 在从成年小鼠睾丸中分离的 RNA 中检测到 1.6 kB mRNA。但不是 从小鼠肝脏中分离出的 RNA 中。脑。心脏或肾脏。 核糖核酸酶 保护分析表明 Tst mRNA 的丰度 在小鼠胚胎发育的第 11 天和第 13 天期间最高。 此外。通过筛选人类，分离出部分人类 FGF-8 cDNA 含有 Tst cDNA 的睾丸 cDNA 文库。