MULTIUSER DESORPTION MASS SPECTROMETER

请拨预算，用于购置MATRIX台式仪器 辅助激光解吸质谱学提供快速 结合蛋白质研究测定分子量 加工、修饰和结构-功能关系。对.的使用 薄膜将被开发为基质辅助激光的表面 解吸，包括解吸酶消化产物和 化学反应直接在膜上进行。协作性 修饰寡核苷酸、多糖类和多糖类的研究 非共价蛋白质复合体将包括这些研究方法的发展 区域。 此安装的目的是在部分时间内运行 无人值守、多用户模式，允许分子生物学家和生物化学家 进行它们自己的分子量测定。培训和证明 方法将先于大多数这些应用程序。

A budget is requested for acquisition of a benchtop instrument for matrix assisted laser desorption mass spectrometry to provide rapid determinations of molecular weights in conjunction with studies of protein processing, modification and structure-function relations. The use of membranes will be developed as surfaces for matrix assisted laser desorption, including desorption of the products of protease digestion and chemical reactions carried out directly on the membrane. Collaborative studies of modified oligonucleotides, of polysaccharides and of noncovalent protein complexes will include methods development in those areas. It is intended that this installation will operate part of the time in a walk-up, multi-user mode, allowing molecular biologists and biochemists to make their own molecular weight determinations. Training and proof of method will precede most of these applications.