LTQ Orbitrap tandem mass spectrometer

LTQ Orbitrap 串联质谱仪

• 批准号: 7211011
• 负责人: Catherine C Fenselau
• 金额: $ 39.37万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7211011
• 关键词: Alkylation; Biological Sciences; Chemicals; Computer software; Disease; Electrospray Ionization; Fostering; Funding Applicant; Heme; High Pressure Liquid Chromatography; Homeostasis; Housing; Isotopes; Maryland; Mass Spectrum Analysis; Nucleic Acids; Peptides; Proteins; Research Personnel; Resolution; Solutions; Universities; base; college; cost; instrument; macrophage; mass spectrometer; multidisciplinary; pathogen; protein structure; response; tandem mass spectrometry

DESCRIPTION (provided by applicant): Funds are requested to acquire an automated tandem mass spectrometer optimized for peptide analysis. The instrument will be unique on our campus, and will provide high pressure liquid chromatography interfaced via nanospray/electrospray ionization, tandem mass spectrometry and MSn capability, product analysis with high resolution and high mass accuracy, software to support peptide and protein identification, the capability for isotope-free quantitation, high sensitivity and high throughput. It will be used by seven major researchers at the University of Maryland to characterize proteins associated with host/pathogen interactions, macrophage responses to diseases, and heme homeostasis; to support studies of protein structures and dynamics in solution; and to develop strategies for targeted alkylation of nucleic acids. The LTQ Orbitrap will be housed in the Mass Spectrometry Facility of the College of Chemical and Life Sciences. This commercially available, technologically sophisticated instrument will support meritorious projects on a shared use, highly cost-effective basis, and foster multidisciplinary interaction.

描述（由申请人提供）：要求提供资金以获得用于肽分析的优化的自动串联质谱仪。该仪器将在我们的校园是独一无二的，并将提供高压液相色谱接口通过nanospray/电喷雾电离，串联质谱和MSn能力，产品分析与高分辨率和高质量的准确性，软件支持肽和蛋白质鉴定，无同位素定量的能力，高灵敏度和高通量。它将被马里兰州大学的七位主要研究人员用于表征与宿主/病原体相互作用、巨噬细胞对疾病的反应和血红素稳态相关的蛋白质;支持溶液中蛋白质结构和动力学的研究;并开发核酸靶向烷基化的策略。LTQ轨道阱将被安置在化学和生命科学学院的质谱设施中。这一商业上可获得的、技术上先进的工具将在共享使用、高成本效益的基础上支持有价值的项目，并促进多学科的互动。