LTQ Orbitrap tandem mass spectrometer

描述(由申请者提供)：为获得优化的多肽分析自动串联质谱计，请提供资金。该仪器在我校将是独一无二的，将提供通过纳米喷雾/电喷雾电离、串联质谱仪和MSN功能进行接口的高压液相色谱、高分辨率和高质量精度的产品分析、支持多肽和蛋白质鉴定的软件、无同位素定量的能力、高灵敏度和高通量。马里兰大学的七名主要研究人员将使用它来表征与宿主/病原体相互作用、巨噬细胞对疾病的反应和血红素动态平衡相关的蛋白质；支持对溶液中蛋白质结构和动力学的研究；以及开发核酸定向烷基化的策略。LTQ轨道探测器将被安置在化学与生命科学学院的质谱学设施中。这种商业上可用的、技术先进的工具将在共享使用、高成本效益的基础上支持有价值的项目，并促进多学科互动。

DESCRIPTION (provided by applicant): Funds are requested to acquire an automated tandem mass spectrometer optimized for peptide analysis. The instrument will be unique on our campus, and will provide high pressure liquid chromatography interfaced via nanospray/electrospray ionization, tandem mass spectrometry and MSn capability, product analysis with high resolution and high mass accuracy, software to support peptide and protein identification, the capability for isotope-free quantitation, high sensitivity and high throughput. It will be used by seven major researchers at the University of Maryland to characterize proteins associated with host/pathogen interactions, macrophage responses to diseases, and heme homeostasis; to support studies of protein structures and dynamics in solution; and to develop strategies for targeted alkylation of nucleic acids. The LTQ Orbitrap will be housed in the Mass Spectrometry Facility of the College of Chemical and Life Sciences. This commercially available, technologically sophisticated instrument will support meritorious projects on a shared use, highly cost-effective basis, and foster multidisciplinary interaction.