LTQ Orbitrap tandem mass spectrometer

描述（由申请人提供）：申请资金用于购买用于肽分析优化的自动串联质谱仪。该仪器将是我们校园中独一无二的，将提供高压液相色谱界面，通过纳米喷雾/电喷雾电离，串联质谱和MSn功能，高分辨率和高质量精度的产品分析，支持肽和蛋白质鉴定的软件，无同位素定量能力，高灵敏度和高通量。它将被马里兰大学的7位主要研究人员用于表征与宿主/病原体相互作用、巨噬细胞对疾病的反应和血红素稳态相关的蛋白质；支持溶液中蛋白质结构和动力学的研究；并制定针对性的核酸烷基化策略。LTQ Orbitrap将被安置在化学和生命科学学院的质谱设备中。这种商业化、技术先进的仪器将在共享使用、高成本效益的基础上支持有价值的项目，并促进多学科的相互作用。

DESCRIPTION (provided by applicant): Funds are requested to acquire an automated tandem mass spectrometer optimized for peptide analysis. The instrument will be unique on our campus, and will provide high pressure liquid chromatography interfaced via nanospray/electrospray ionization, tandem mass spectrometry and MSn capability, product analysis with high resolution and high mass accuracy, software to support peptide and protein identification, the capability for isotope-free quantitation, high sensitivity and high throughput. It will be used by seven major researchers at the University of Maryland to characterize proteins associated with host/pathogen interactions, macrophage responses to diseases, and heme homeostasis; to support studies of protein structures and dynamics in solution; and to develop strategies for targeted alkylation of nucleic acids. The LTQ Orbitrap will be housed in the Mass Spectrometry Facility of the College of Chemical and Life Sciences. This commercially available, technologically sophisticated instrument will support meritorious projects on a shared use, highly cost-effective basis, and foster multidisciplinary interaction.