AUTOMATED DNA SEQUENCING INSTRUMENTATION

自动化 DNA 测序仪器

• 批准号: 2284253
• 负责人: Judith L CAMPBELL
• 金额: $ 20万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-06 至 1995-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284253
• 关键词: 2'3' dideoxynucleoside; animal genetic material tag; biomedical automation; biomedical equipment purchase; computer assisted sequence analysis; computer program /software; fluorescent dye /probe; nucleic acid sequence; oligonucleotides; personal computers

We request funding for instrumentation to be used in automated DNA sequencing. We have determined that a centralized sequencing facility will save considerable time and consumable costs for the 19 major users and others in the chemistry and biology divisions at Caltech, while at the same time contributing data crucial to the success of the projects. Large amounts of sequencing are carried out in the day to day projects, and it all must be done by manual methods based on radioactivity by individual students, post-doctoral fellows and technicians, many of whom have no previous experience in DNA sequencing. This leads to lack of efficiency and loss of competitiveness. We are requesting three items of related equipment: an automated DNA sequencer that runs and reads electrophoresis gels, a robotic station to carry out the polymerization reactions on which DNA sequencing technology is based, and computer hardware and software to update an existing Sequencing Analysis Facility to make it able to cope with the increased load that will arise with the advent of the automated sequencing facility here. Automated sequencing based on fluorescent labels has at least four advantages over manual methods based on radioactivity: saving in consumable costs, labor saving, safety, and, most important, quality of results. Perhaps the most useful aspect of a central sequencing facility based on automated methods is the extent to which the automated sequence can be integrated with computer systems. The facility will, thus, enhance the productivity and quality of the studies of the major users. There are 19 major users documented here and additional users are mentioned. The laboratories houses over 300 investigators, students, post-doctoral fellows and technicians. The major applications of the instruments will be projects that involve extensive cloning and sequencing of novel genes and/or construction of genetically engineered constructs that require sequence analysis. The projects cover, among others, sequencing of genes involved in RNA splicing, in molecular control of neural crest development, in the survival and differentiated function of neurons in Drosophila, in membrane excitability, in controlling body segment development in Drosophila, in cell cycle regulation, in DNA replication in yeast, in cell fate and morphogenesis in several organisms. One project involves sequencing large numbers of randomly-chosen cDNA clones involved in floral development in Arabidipsis.

我们要求用于自动DNA的仪器资金 测序。 我们已经确定了集中测序设施 将为19个主要用户节省大量时间和消耗的成本 以及加州理工学院化学和生物学分界的其他人 同一时间贡献了对项目成功至关重要的数据。 在日常项目中进行大量测序， 所有这些都必须通过基于放射性的手动方法来完成 个人学生，博士后研究员和技术人员，其中许多 没有以前的DNA测序经验。 这导致缺乏 效率和竞争能力的丧失。 我们要求三个项目 相关设备：运行和读取的自动DNA音序器 电泳凝胶，一个机器人站，用于进行聚合 DNA测序技术所基于的反应，计算机 硬件和软件以更新现有的测序分析设施 为了使其能够应对将增加的负载 自动测序设施的出现。 自动测序 基于荧光标签至少具有四个优点 基于放射性的方法：节省消耗成本，节省人工， 安全，最重要的是结果的质量。 也许是最有用的 基于自动化方法的中央测序设施的方面是 自动序列可以与计算机集成的范围 系统。 因此，该设施将提高生产率和质量 主要用户的研究。 这里有19个主要用户，其他用户是 提及。 实验室设有300多名调查员，学生， 博士后研究员和技术人员。 主要应用 仪器将是涉及广泛克隆和的项目 新基因的测序和/或基因工程的结构 需要序列分析的构造。 项目涵盖， 其他，分子中涉及RNA剪接的基因的测序 控制神经rest发育，生存和分化 果蝇中神经元的功能，膜兴奋性，在 在细胞周期中控制果蝇的身体片段发展 调节，在酵母中的DNA复制中，细胞命运和形态发生 在几种生物中。 一个项目涉及对大量的测序 参与花卉发育的随机选择的cDNA克隆 拟南芥。