GENETIC DEVELOPMENT OF SPECIFIC MUSCLE DEVELOPMENT IN DROSOPHILA

果蝇黑腹果蝇的无果基因将是 从基因上分析，结合了各种分子和解剖学 技巧。根据先前的分析，这个基因座控制着各种男性- 特定的、与性行为相关的功能；它在 然而，基因表达完全没有特征性。一无所获的人 这里要研究的函数是这个轨迹在 对特定发生的大肌肉发育的调节 在成年男性腹部。一种方法是诱导和隔离新的 破坏这一功能的突变；“诱变剂”将是一种 标记的转座子，有助于直接克隆无果DNA 与镶件相邻。这可能导致克隆和分子 对徒劳的刻画。一套独立的实验将 评估这些新突变对神经肌肉的表型影响 解剖学：腹部肌肉组织及其神经将 具有变异肌肉和正常肌肉的特征。一种关联 类似的实验将评估腹部是一部分的男性马赛克 正常，部分无果。这项实验有可能 确定无果是否编码了一种“发育诱导剂” 调节这块肌肉的发育。这些实验将暴露 学生学习遗传逻辑，并教授遗传变异在 推断男性专一性发育的基因功能 肌肉。

The fruitless locus in the fruit fly Drosophila melanogaster will be analyzed genetically, incorporating a variety of molecular and anatomical techniques. By prior analysis, this locus controls a variety of male- specific, sex-behavior related functions; its function at the level of gene expression, however, is completely uncharacterized. The fruitless function that will be studied here is the role this locus plays in the regulation of the development of a large muscle that occurs specifically in adult male abdomens. One approach will be to induce and isolate new mutations that upset this function; the "mutagenic agent" will be a marked transposon which will facilitate direct cloning of fruitless DNA contiguous with the insert. This could lead to cloning and molecular characterization of fruitless. An independent set of experiments will assess the phenotypic impact of these new mutations on neuromuscular anatomy; the abdominal musculature and its innervation will be characterized in both mutant and normal muscles. A correlating experiment will similarly assess male mosaics whose abdomens are part normal, part fruitless in genotype. This experiment could potentially determine whether fruitless encodes a "developmental inducer" which regulates the development of this muscle. These experiments will expose students to genetic logic, and teach the utility of genetic variants in deducing gene function underlying the development of the male-specific muscle.