AQUATIC BIOAVAILABILITY OF PAHS, PCB, AND PCDDS/PCDFS IN THE MASSENA AREA

以往对马塞纳环境污染的研究 圣劳伦斯河地区的危险废物集中在 通用汽车公司在马塞纳附近的工厂。然而，有两个 该地区的铝制造厂和这些工厂的排放物 这些设施已经向河流排放了污染物。的主要目标是 该建议是为了确定多环化合物的水生生物利用度。 芳香烃(PAHs)、多氯联苯(PCbs)和 多氯二苯并对二恶英S/多氯二苯并呋喃 从每个工厂排污点附近收集的沉积物中提取多氯二苯并呋喃。 这项任务将通过暴露蚊子幼虫摇蚊来完成 触须和笼养鲤鱼对实验室和田间沉积物的影响 分别进行了分析。此外，鲤鱼和航前幼体来自两个物种 涉水鸭子(gadwall和绿头鸭)将在 每个厂址和一个控制点。使用最先进的气体 色谱仪(GC)/高分辨率质谱仪(MS)，应该是 可以检测到1皮克(Pg)的单个化合物 样品(脂肪、肝脏、幼虫、昆虫和沉积物)的提取物。 拟议研究项目的次要目标将是 确定新陈代谢对选择性保留的影响 污染物。一般来说，生物群只积累有毒的2，3，7，8-取代 多氯二苯并二恶英，以及在多氯二苯并呋喃的情况下，少量的非2，3，7，8- 取代异构体。然而，马塞纳地区的许多鱼类和野生动物 发现样品中含有较高数量的非2，3，7，8-取代 多氯二苯并呋喃比2，3，7，8-取代异构体。来检验这一假设 代谢影响多氯二苯并二恶英/多氯二苯并呋喃及其相关化合物的保留 鲤鸭肝脏细胞色素P450酶活性的研究 样品将使用芳烃羟基酶(AHH)进行测定。 化验。 这项研究的结果应该有助于更好地理解 个别行业对鱼类和鱼类污染的相对贡献 马塞纳地区居民食用的野生动物。

Previous studies carried out on environmental contamination int he Massena area of the St. Lawrence River have focused on hazardous wastes from the General Motors Corporation plant near Massena. However, there are two aluminum manufacturing plants in the area and discharges from these facilities have released pollutants to the river. The major objective of this proposal is to determine the aquatic bioavailability of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins (PCDD)s/polychlorinated dibenzofurans (PCDFs) from sediments collected near discharge points from each plant. This task will be accomplished by exposing the midge larva, Chironomus tentans, and caged-carp to sediments in the laboratory and in the field respectively. In addition carp and preflight juveniles from two species of dabbling ducks (gadwalls and mallards) will be collected in the vicinity of each plant site and at a control site. Using a state-of-the-art gas chromatograph (GC)/high resolution mass spectrometer (MS), it should be possible to detect 1 picogram (pg) amounts of individual compounds in extracts from samples (fat, liver, larvae, insects and sediments). The secondary objective of the proposed research project will be to determine the influence of metabolism on selective retention of the pollutants. Generally biota accumulate only toxic 2,3,7,8-substituted PCDDs and also, in the case of PCDFs, small quantities of non-2,3,7,8- substituted isomers. However many of the Massena area fish and wildlife samples were found to contain higher quantities of non-2,3,7,8-substituted PCDFs than 2,3,7,8-substituted isomers. To test the hypothesis that metabolism influences the retention of PCDDs/PCDFs and related compounds the activity of hepatic Cytochrome P450 enzymes in the carp and duck samples will be determined using the aryl hydrocarbon hydroxylase (AHH) assay. Results from this study should provide for a better understanding of the relative contributions of individual industries to pollution of fish and wildlife consumed by residents of the Massena area.