Genetic analysis in the family Cyprinidae base on isozymes and mtDNA

基于同工酶和mtDNA的鲤科遗传分析

• 批准号: 63560179
• 负责人: KIJIMA Akihiro
• 金额: $ 1.47万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560179/
• 关键词: mtDNA; isozyme; Cyprinidae; crucian carp; population structure; genetic divergence; コイ科魚類; フナ属; フナ属魚類; 遺伝様式

There are many kind of fish species in the family cyprinidae, and these species evolved from allo-tetraploid fish species. Then, they could be constructed a complex genetic feature and genetic structure. Isozymic analyses are very useful tool for analyzing a population structure, genetic divergence and evolutionary feature, but they could analyze a long generations.Mitochondrial DNA (mtDNA) has been expected to use as sensitive genetic markers for fish genetics and fishery management. However, in order to apply it for actual population study, a lot of issues should be resolved.The aims in the present study are (1) establishment an elementary method for purifying mtDNA in freshwater fish, (2) Clarifying a value of restriction fragment length polymorphism in carp by use a genetic marker of mtDNA, and (3) identification of species of crucian carp inhabited in Tohoku area in Japan.As the results, the elementary method for purifying mtDNA could be established based on chemical methods (alkarine treatment and non-idet P-40). Next, based on that method, restriction fragment length polymorphisms were observed in the four restriction enzymes, and then the 4 maternal strains are estimated in carp inhabited in a certain pond. Furthermore, the three sub species of crucian carp have existed in the Tohoku area, and genetically divergent populations have been inhabited and brood in a pond. However, mtDNA variant patterns were common in both populations.

鲤科鱼类种类繁多，这些鱼类均由异源四倍体鱼类进化而来。然后，可以构建一个复杂的遗传特征和遗传结构。同工酶分析是分析种群结构、遗传分化和进化特征的有效工具，但其分析世代较长，线粒体DNA（mitochondrialDNA，mtDNA）有望作为敏感的遗传标记用于鱼类遗传学和渔业管理。本研究的目的是建立淡水鱼mtDNA的初步纯化方法，利用mtDNA遗传标记对鲤鱼限制性片段长度多态性进行分析，并对日本东北地区的鲫鱼进行鉴定。在化学法（碱性磷酸酶法和非idet P-40法）的基础上，初步建立了纯化mtDNA的方法。然后，基于该方法，在四种限制性内切酶中观察限制性片段长度多态性，然后在某池塘中栖息的鲤鱼中估计4个母本菌株。此外，东北地区存在着三个亚种的鲫鱼，遗传上不同的种群已经在池塘中栖息和繁殖。然而，mtDNA变异模式在两个群体中是常见的。

项目成果

木島明博,谷口順彦,坂東喜久雄,向井龍男: "アルカリ処理法によるウナギ、コイ、アユのミトコンドリアDNA(mtDNA)の単離と塩基対数の測定" 水産育種. 15. 51-57 (1990)

Akihiro Kijima、Yoshihiko Taniguchi、Kikuo Bando、Tatsuo Mukai：“通过碱处理方法分离鳗鱼、鲤鱼和香鱼的线粒体 DNA (mtDNA) 和碱基对数的测量”渔业育种 15. 51-57 (1990)。

Kijima, A., N.Taniguchi, K.Bando, T.Mukai: "Rapid isolation of mitochondrial DNA by alkarine treatment and estimation of the number of their base pairs in Japanese eel, carp, and ayu" Fish Genet. Breed. Sci.15. 51-57 (1990)

Kijima, A.、N.Taniguchi、K.Bando、T.Mukai：“通过碱处理快速分离线粒体 DNA 并估计日本鳗鱼、鲤鱼和香鱼中碱基对的数量”《鱼类基因》。