Researching the role of dissolved organic matter as a nutrient resource in freshwater ecosystems

淡水生物多样性的丧失速度比地球上任何其他地区都要快。一系列广泛的压力因素正在推动这一趋势，其中气候变化和粮食生产和消费增加的营养输送无处不在。由于在快速变化的环境中进行实验的物理挑战，以及对生物可利用的养分形式的狭隘认识，迄今为止关于养分富集对淡水生物区系影响的研究受到限制。它侧重于特定物种对无机营养物形式的反应，通常是在体外或湖泊中，很少用于环境条件下的流动沃茨，通常用于微生物或浮游生物，忽略了其他生物群体的反应，群落水平对富集的反应，以及淡水沃茨中生物可利用有机化合物范围的综合影响。需要转型研究，以更新目前的营养循环理论流生态系统，从研究解释如何生态系统的一部分，以有限的范围内的压力源，基本的，整体的理论解释整个生态系统如何应对广泛的调色板的压力。我将领导一个多学科团队来应对这一挑战，在野外和气候变化的条件下，应用分子尺度分析、稳定同位素探测和环境基因组学方面的创新技术。然后，我们将使用新的数据驱动的建模来了解分类和功能变化之间的关系，以响应溶解有机质（DOM）和无机营养素暴露，揭示环境x基因在生物对营养和气候压力的反应中的相互作用。这将推进当前的理论，并改变我们对淡水生态系统的全面营养组合的影响的理解，揭示DOM的具体作用，因为这是根据DOM池的组成，生态系统的物种组成，流stoichoimetry和环境特征。

Freshwaters are losing biodiversity at a higher rate than any other planetary domain. A wide range of stressors are driving this trend, of which climate change and increasing nutrient delivery from food production and consumption are ubiquitous. Research to date on nutrient enrichment impacts on freshwater biota has been limited by the physical challenge of experimentation in a rapidly changing environment, and a narrow perception of bioavailable nutrient forms. It has focused on species-specific responses to inorganic nutrient forms, often in vitro or in lakes, rarely for flowing waters under ambient conditions, and usually for microbial or planktonic organisms, ignoring the responses of other biotic groups, community level responses to enrichment, and the combined impacts of the range of bioavailable organic compounds in freshwaters. Transformational research is needed to update current nutrient cycling theory for stream ecosystems, shifting from research explaining how part of the ecosystem responds to a limited range of stressors, to fundamental, holistic theory explaining how whole ecosystems respond to a broad palette of stressors. I will lead a multidisciplinary team to tackle this challenge, applying innovative techniques in molecular scale analysis, stable isotope probing and environmental genomics, under field and climatically-altered conditions. We will then use new data-driven modelling to understand relationships between taxonomic and functional shifts in response to dissolved organic matter (DOM) and inorganic nutrient exposure, revealing environment x gene interactions in biotic responses to nutrient and climate stressors. This will advance current theory and transform our understanding of the impacts of the full nutrient portfolio on freshwater ecosystems, revealing the specific role of DOM as this varies according to the composition of the DOM pool, species composition of the ecosystem, stream stoichoimetry and environmental character.