MOLECULAR STUDIES OF EUKARYOTIC GENE REGULATION

真核基因调控的分子研究

• 批准号: 3774297
• 负责人: B M PATERSON
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774297
• 关键词: Drosophilidae; cell differentiation; chickens; developmental genetics; eukaryote; gene expression; genetic regulation; muscle cells; muscle proteins; myoblasts; myogenesis; phosphorylation; protein structure function; transcription factor

We have continued our studies on the genes involved in cellular commitment of the myoblast and its subsequent differentiation during myogenesis. The precursor myoblast is defined early in development within the somite compartment as a group of cells expressing the MyoD family of gene regulatory factors, MyoD, myogenin, myf5, and MRF4. The expression of these factors not only marks the committed cell but also plays a role in activating the muscle-specific genes during terminal differentiation. We have isolated these four genes in the chicken and have been studying their pattern of expression in development, both in vivo and in vitro, determining the role of phosphorylation in their functional regulation, and identifying other related bHLH proteins that interact with these myogenic factors to regulate their function. In order to take a genetic approach to the study of myogenesis we have also isolated the MyoD homolog in Drosophila where it is a single gene that marks a subset of muscle precursor cells. By studying the Drosophila gene, the proteins that regulate its function, and the upstream and downstream genes in the myogenic pathway in the fly, we hope to identify correlates in the vertebrates to help us understand more completely vertebrate myogenesis.

我们继续研究涉及细胞承诺的基因 肌细胞及其在肌发生过程中的随后分化。 这 前体肌细胞在体内的开发早期定义 作为表达基因MYOD家族的一组细胞的隔室 调节因素，Myod，Myogenin，MyF5和MRF4。 表达 这些因素不仅标志着所授权的单元，而且还在 在终末分化过程中激活肌肉特异性基因。 我们 已经在鸡中隔离了这四个基因，并且一直在研究它们 在体内和体外的发育中表达模式， 确定磷酸化在其功能调节中的作用， 并确定与这些相互作用的其他相关BHLH蛋白 肌原因素调节其功能。 为了采用遗传 研究肌发生的方法我们也隔离了Myod同源物 在果蝇中，它是一个标记肌肉子集的单个基因 前体细胞。 通过研究果蝇基因，即蛋白质 调节其功能，以及上游和下游基因 肌源性途径中，我们希望识别 脊椎动物帮助我们更完全理解脊椎动物的肌发生。