TISSUE CULTURE GROWTH FACTOR STUDY--METABOLIC DISORDERS

组织培养生长因子研究——代谢紊乱

• 批准号: 3095501
• 负责人: WALLACE LEE MCKEEHAN
• 金额: $ 91.46万
• 依托单位: ADIRONDACK BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3095501
• 关键词: cell differentiation; cell growth regulation; growth factor; hormone regulation /control mechanism; tissue /cell culture

Cellular endocrinology/paracrinology/autocrinology is the science of dissection and reconstitution of isolated cells and their environment in order to understand the physiology of the regulation of growth, function and differentiation. This knowledge applied to particular cell types that compose particular tissues is essential to understand and intervene in metabolic disease associated with specific tissues. This program seeks to understand the metabolic basis of. disease by application of these cellular level principles with modern protein chemical, molecular biological and genetic approaches The program is composed of four interrelated, but novel, projects which address specific questions concerning growth, function and differentiation of vascular, liver, adipose and thymic tissues. Two projects consider detailed molecular problems concerning the nature of the receptor, the proximal signal generated by factor (vascular endothelial cell growth factor and heparin-binding fibroblast growth factor)-receptor interaction and the control of the resulting signal. Two projects explore the integration of the EGF-receptor kinase interaction with the phospholipase A2/prostaglandin signal pathway in vitro and in vivo. The projects are backed by state-of-the-art protein chemistry and molecular core laboratories, the latter of which includes proposed establishment of a transgenic animal facility.

细胞内分泌学/旁分泌学/自分泌学是 分离的细胞及其环境的解剖和重建， 为了了解生理学的调节生长，功能 和差异化。 这些知识适用于特定的细胞类型 组成特定组织的基因对于理解和干预 与特定组织相关的代谢疾病。 这个程序 试图了解的代谢基础。疾病的应用 这些细胞水平的原则与现代蛋白质化学，分子 生物学和遗传学方法该计划由四个组成 解决具体问题的相互关联但新颖的项目 涉及血管，肝脏， 脂肪和胸腺组织。 两个项目考虑详细的分子 关于受体性质的问题，近端信号 由因子（血管内皮细胞生长因子和 肝素结合成纤维细胞生长因子）-受体相互作用和 控制产生的信号。 两个项目探讨了 EGF受体激酶与磷脂酶的相互作用 A2/前列腺素信号通路的体外和体内研究。 这些项目 由最先进的蛋白质化学和分子核心 实验室，后者包括拟议建立一个 转基因动物设施。