MAMMALIAN GENES ENCODING ENHANCER OF SPLIT GROUCHO PROTEIN

编码分裂格罗乔蛋白增强子的哺乳动物基因

• 批准号: 3755473
• 负责人: S L STEVEN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755473
• 关键词: RNA splicing; animal genetic material tag; clone cells; complementary DNA; gene expression; genetic enhancer element; genetic mapping; genetic transcription; hamsters; human genetic material tag; human tissue; laboratory mouse; northern blottings; nucleic acid sequence; structural genes; transcription factor

Mouse and human cDNAs encoding AES (Amino Enhancer of Split) and ESG (Enhancer of Split Groucho) proteins with strong homology to Drosophila Enhancer of split groucho protein were isolated and sequenced. Mouse AES-1 and AES-2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino-terminal region of Drosophila groucho, mouse ESG and human TLE (Transducin-Like Enhancer of split) proteins. Mouse AES transcripts of 1.5Kb and 1.2Kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 Kb and 1.4Kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3Kb and 4.0Kb were found only in testis, while human TLE 1 transcripts of 4.5Kb were more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosome no. 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.

编码AES（氨基分裂增强子）和ESG的小鼠和人cDNA 与果蝇具有强同源性的（分裂Groucho增强子）蛋白 分离分裂groucho蛋白的增强子并测序。 鼠标 AES-1和AES-2蛋白质，可能由选择性剪接产生， 分别含有202和196个氨基酸，而小鼠ESG蛋白 由771个氨基酸组成。 小鼠和小鼠的氨基酸序列 发现人AES蛋白与人AES蛋白具有约50%的同一性， 果蝇、小鼠和人ESG氨基末端区 TLE（Transducin-Like Enhancer of split）蛋白。 小鼠AES 1.5Kb和1.2Kb的转录本在肌肉中大量表达， 心脏和大脑 1.6Kb和1.4Kb的人AES转录本， 主要存在于肌肉、心脏和胎盘中。 小鼠ESG TLE 3基因的同源物，仅发现3.3Kb和4.0Kb的转录本 而人TLE 1基因4.5Kb的转录本在睾丸中的表达量较高， 与心脏、大脑、肺、肝脏、肾脏和 胰腺 将人AES、TLE 1和TLE 3基因定位于染色体上 号19，9和15，分别使用人和中国仓鼠杂交 细胞系