MAMMALIAN GENES ENCODING ENHANCER OF SPLIT GROUCHO PROTEIN

编码分裂格罗乔蛋白增强子的哺乳动物基因

• 批准号: 5202239
• 负责人: S L STEVEN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5202239
• 关键词: Drosophilidae; complementary DNA; ectoderm; genetic enhancer element; genetic mapping; in situ hybridization; laboratory mouse; neurogenesis; northern blottings; nucleic acid sequence; spermatogenesis; structural genes; transcription factor

In the fruitfly Drosophila melanogaster, neurogenesis is under the control of several loci, including Enhancer of split, whose products appear to determine the fate of neuroectodermal cells during the embryo development. These genes appear to play significant roles in spermatogenesis in addition to neurogenesis. The cDNAs encoding the enhancer of split groucho (ESG) and its related amino-terminal (AES) proteins were isolated and sequenced from human, mouse and Xenopus. The ESG proteins consist of 771 amino-acids, while the AES proteins contain 197 amino-acids. The AES proteins were found to exhibit approximately 50% identity to the amino-terminal region of Drosophila enhancer of split groucho protein. Human AES and two ESG genes have been mapped using human and Chinese hamster hybrid cell lines to chromosomes 19, 9 and 15, respectively. Human AES gene was further localized by fluorescence in- situ hybridization to chromosome band 19p13.3, and its protein-encoding sequence is interrupted by six introns. The evolutionary relationships among these AES and ESG genes were analyzed. The physiological function(s) of vertebrate AES and ESG proteins during spermatogenesis, oogenesis, and embryogenesis, including neurogeneis, are being investigated.

在果蝇果蝇中，神经发生是在 控制多个位点，包括分裂增强子，其产物 似乎决定了胚胎期间神经外胚层细胞的命运 发展。这些基因似乎在以下方面发挥着重要作用： 除了神经发生之外还有精子发生。编码 cDNA 的 split groucho 增强子 (ESG) 及其相关氨基末端 (AES) 从人类、小鼠和非洲爪蟾中分离出蛋白质并进行测序。这 ESG 蛋白由 771 个氨基酸组成，而 AES 蛋白包含 197 个氨基酸。发现 AES 蛋白表现出大约 50% 与果蝇分裂增强子氨基末端区域的同一性 格劳乔蛋白。人类 AES 和两个 ESG 基因已使用人类绘制 以及19、9和15号染色体的中国仓鼠杂交细胞系， 分别。人类 AES 基因通过荧光进一步定位 染色体带 19p13.3 的原位杂交及其蛋白质编码 序列被六个内含子打断。进化关系 对这些 AES 和 ESG 基因进行了分析。生理学 脊椎动物 AES 和 ESG 蛋白在精子发生过程中的功能， 卵子发生和胚胎发生，包括神经发生，正在被 调查了。