ANATOMICAL ANALYSIS OF NEURONAL CIRCUITS

这个项目旨在提高我们对详细信息的理解 丘脑和它的回路。与唤醒的关系，圆滑- 觉醒周期和皮层下继电器的信息传输 到大脑皮层。最近的证据表明，网状结构 丘脑网状核对脑内神经系统发育的影响 产生一些有节奏的振荡。它们是前脑的特征 活动在慢波中流畅。这项研究的重点是 识别可能影响兴奋性的传入输入 丘脑网状核内的神经元，并确定 这些传入神经与细胞体的关系以及 丘脑网状核神经元的树突。 在过去的一年里，外部苍白球投射在 对松鼠猴丘脑网状核进行了电子显微镜观察 外段注射WGA-HRP后的显微镜观察 苍白球和包埋后GABA的免疫组织化学研究 使用胶体金。最常见的GABA能突触轮廓 猕猴丘脑网状核内有较大的突起 充满了许多多形性小泡和线粒体。这些 Boutons对GABA进行了密集的染色，总是突触前，并建立 丘脑胞体和树突上的对称性突触接触 网状核神经元。少量的GABA能谱图 在丘脑中也发现参与了一系列突触 网状核神经纤维层。剖面内的GABA染色 参与串联突触的能力较弱，它们包含的囊泡较少 和线粒体，而不是F末端。外部苍白球 用HRP标记的终末来自有稀疏髓鞘的轴突， 类似于F端子；即。它们很大，挤满了许多多形性的 囊泡和线粒体总是突触前，浓染 GABA，并在体细胞上频繁地建立对称突触 丘脑网状核神经元的近侧树突。详细信息： 这些与已知的其他外在GABA能输入之间的关系 进入丘脑网状核尚待检查。

This project seeks to improve our understanding of the detailed circuitry of the thalamus and it. relation to arousal, sleek- wakefulness cycles, and information transfer from subcortical relays to the cerebral cortex. Recent evidence suggests that the reticular nucleus of the thalamus reticular nucleus is critical for the generation of some rhythmic oscillation. which characterize forebrain activity during slow-wave sleek. The focus of this study is to identify the afferent inputs which might influence the excitability of neurons within the thalamic reticular nucleus and to determine the details of the relationship of these afferents to the cell bodies and dendrites of thalamic reticular nucleus neurons. During the past year, the external pallidal projection upon the squirrel monkey thalamic reticular nucleus was examined in the electron microscope following injections of WGA-HRP into the external segment of the globus pallidus and post-embedding immunohistochemistry for GABA using colloidal gold. The most common GABAergic synaptic profiles within the monkey thalamic reticular nucleus neuropil are large boutons packed with many pleomorphic vesicles as well as mitochondria. These boutons stain densely for GABA, are-always presynaptic, and establish symmetric synaptic contacts upon the somata and dendrites of thalamic reticular nucleus neurons. A small number of GABAergic profiles which participate in serial synapses was also found within the thalamic reticular nucleus neuropil. The GABA staining within profiles which participate in serial synapses is weak and they contain fewer vesicles and mitochondria than the F terminals. The external pallidal terminals, labeled with HRP, arise from thinly myelinated axons, and resemble F terminals; viz. they are large, packed with many pleomorphic vesicles and mitochondria, are always presynaptic, stain densely for GABA, and establish symmetric synapses frequently upon the somata and proximal dendrites of thalamic reticular nucleus neurons. Details of the relation of these to other extrinsic GABAergic inputs known to enter the thalamic reticular nucleus remain to be examined.