The development of animal model using squirrel monkey (Saimiri sciureus boliviensis) for human malaria infection

利用松鼠猴（Saimiri sciureus boliviensis）开发人类疟疾感染动物模型

• 批准号: 15500296
• 负责人: HATTORI Shosaku
• 金额: $ 2.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500296/
• 关键词: squirrel monkey; malaria; measles virus; 実験動物; 零長類; 新世界ザル; フラーサイトメトリー

Squirrel monkeys are invaluable experimental animals in research of human infection diseases such as malaria parasites. However, the number of monoclonal antibodies (mAb) available against immunological studies using this monkey is limited. Thus, we screened 45 anti-human mAbs for cross-reactivity with peripheral blood mononuclear cells of this primate by flow cytometry analysis. Twenty-four antibodies of 45 anti-human mAbs tested were cross-reacted with surface antigens of squirrel monkey cells. Fifteen antibodies of 24 mAbs cross-reacted with monkey cells were found newly in this study. With the use of some of mAbs cross-reacted with the Saimiri cells we found the age-related changes in the proportion of lymphocyte subsets of peripheral blood. With increasing age, the proportion of B cells declined, whereas the percentage of T cells rose. In addition, there was an inverse correlation between CD4 single positive and CD8 single positive T cells. Furthermore, we found that the percentage of double-positive T cells which expressed both CD4 and CD8 molecules increased with age.

松鼠猴是研究疟疾等人类传染病的重要实验动物。然而，使用该猴进行免疫学研究的单克隆抗体（mAb）数量有限。因此，我们通过流式细胞术分析筛选了45种抗人mAb与该灵长类动物外周血单核细胞的交叉反应性。检测的45株抗人单克隆抗体中有24株与松鼠猴细胞表面抗原发生交叉反应。在24株mAb中，新发现15株抗体与猴细胞发生交叉反应。用与Saimiri细胞发生交叉反应的单克隆抗体检测外周血淋巴细胞亚群的比例，发现其随年龄的变化。随着年龄的增长，B细胞的比例下降，而T细胞的百分比上升。此外，CD4单阳性和CD8单阳性T细胞之间存在负相关。此外，我们发现，双阳性T细胞的百分比，同时表达CD4和CD8分子的增加与年龄。

项目成果

Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4.

• DOI: 10.1016/j.cimid.2004.03.011
• 发表时间: 2004-11
• 期刊: Comparative immunology, microbiology and infectious diseases
• 作者: A. Nuntaprasert;Y. Mori;K. Fujita;M. Yoneda;R. Miura;K. Tsukiyama-Kohara;C. Kai

Prevalence of canine distemper virus, feline immunodeficiency virus and feline leukemia virus in captive African lions (Panthera leo) in Japan

• DOI: 10.1292/jvms.66.1587
• 发表时间: 2004-12-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Endo, Y;Uema, M;Kai, C
• 通讯作者: Kai, C

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