The development of animal model using squirrel monkey (Saimiri sciureus boliviensis) for human malaria infection

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Squirrel monkeys are invaluable experimental animals in research of human infection diseases such as malaria parasites. However, the number of monoclonal antibodies (mAb) available against immunological studies using this monkey is limited. Thus, we screened 45 anti-human mAbs for cross-reactivity with peripheral blood mononuclear cells of this primate by flow cytometry analysis. Twenty-four antibodies of 45 anti-human mAbs tested were cross-reacted with surface antigens of squirrel monkey cells. Fifteen antibodies of 24 mAbs cross-reacted with monkey cells were found newly in this study. With the use of some of mAbs cross-reacted with the Saimiri cells we found the age-related changes in the proportion of lymphocyte subsets of peripheral blood. With increasing age, the proportion of B cells declined, whereas the percentage of T cells rose. In addition, there was an inverse correlation between CD4 single positive and CD8 single positive T cells. Furthermore, we found that the percentage of double-positive T cells which expressed both CD4 and CD8 molecules increased with age.