FLUID AND MACROMOLECULAR EXCHANGE IN LUNG

肺中的液体和大分子交换

• 批准号: 3780004
• 负责人: ROBERT J ROSELLI
• 金额: --
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3780004
• 关键词: adult respiratory distress syndrome; biological fluid transport; computer data analysis; disease /disorder model; gel electrophoresis; hyaluronate; interstitial; iodine; lung; lung injury; lymph; mathematical model; perfusion; protein transport; pulmonary circulation; radionuclide imaging /scanning; radionuclides; respiratory airway pressure; technetium; vascular endothelium permeability

The objectives of this project are to continue to develop and test theories of blood-tissue-lymph fluid and macromolecule transport, and to apply these to measurements of lung transvascular exchange. We shall examine critically the assumptions of our current theory and evaluate its utility in the interpretation of complex injuries to the lung vasculature and the actions of therapies aimed at preventing or reversing those injuries. We shall extend the theory so that it can be used to analyze protein flux data which has been collected using a minimally invasive external detection system, intended to simulate potential clinical applications of the method, and validate the method by direct comparison with lymph data. Various methods of normalizing transport coefficients computed from externally detected transvascular protein flux will be compared with normalization by dry lung weight. We propose to examine the importance of solute and membrane electrical charge on the transcapillary movement of fluid and solutes. We will seek specific additions and enhancements to our current methods of protein analysis so that we can increase the number of different proteins analyzed, and improve the precision of each measurement. In addition we will measure interstitial volume, protein excluded volume, and the fraction of lung lymph which passes through the cannulated lymphatic, enabling us to study how these factors affect the dynamics of fluid and macromolecular exchange.

该项目的目标是继续开发和测试 血液-组织-淋巴液-大分子学说 运输，并将其应用于肺的测量 经血管交换 我们将批判性地审视 假设我们目前的理论，并评估其效用， 解释肺血管系统的复杂损伤， 旨在预防或逆转这些疾病的治疗措施， 受伤 我们将扩展这一理论，以便能用于 分析蛋白质通量数据，该数据已经使用 微创外部检测系统，预期用于 模拟该方法的潜在临床应用，以及 通过与淋巴结数据的直接比较，验证了该方法的有效性。 计算的传输系数归一化的各种方法 从外部检测到的经血管蛋白通量将是 与肺干重正常化相比。 我们建议 检查溶质和膜电的重要性 液体和溶质的跨毛细血管运动的电荷。 我们 将寻求具体的补充和增强，以我们目前的 蛋白质分析的方法，这样我们就可以增加 分析不同蛋白质，并提高每个蛋白质的精确度。 测量. 此外，我们将测量间隙体积， 蛋白质排除体积，和肺淋巴液的分数， 通过淋巴管，使我们能够研究 这些因素如何影响流体的动力学， 大分子交换