MECHANISM FOR BLOOD-TISSUE-LYMPH EXCHANGE OF FLUID, MACROMOLECULES IN LUNG

肺中液体、大分子的血液-组织-淋巴交换机制

• 批准号: 3858692
• 负责人: ROBERT J ROSELLI
• 金额: --
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858692
• 关键词: adult respiratory distress syndrome; antipyrine; biological fluid transport; blood osmolarity; capillary; computer simulation; electrical potential; electron density; endotoxins; extracellular; gel electrophoresis; indicator dilution test; lung; macromolecule; mathematical model; membrane permeability; microcirculation; protein transport; pulmonary circulation; pulmonary edema; radionuclide double label; radionuclide imaging /scanning; sheep; solute; technetium; vascular endothelium permeability

The objectives of this project are to continue to develop and test theories of blood-tissue-lymph fluid and macromolecule transport, and to apply these to measurements of lung transvascular exchange. We shall examine critically the assumptions of our current theory and evaluate its utility in the interpretation of complex injuries to the lung vasculature and the actions of therapies aimed at preventing or reversing those injuries. We shall extend the theory so that it can be used to analyze protein flux data which has been collected using a minimally invasive external detection system, intended to simulate potential clinical applications of the method, and validate the method by direct comparison with lymph data. Various methods of normalizing transport coefficients computed from externally detected transvascular protein flux will be compared with normalization by dry lung weight. We propose to examine the importance of solute and membrane electrical charge on the transcapillary movement of fluid and solutes. We will seek specific additions and enhancements to our current methods of protein analysis so that we can increase the number of different proteins analyzed, and improve the precision of each measurement. In addition we will measure interstitial volume, protein excluded volume, and the fraction of lung lymph which passes through the cannulated lymphatic, enabling us to study how these factors affect the dynamics of fluid and macromolecular exchange.

该项目的目标是继续开发和测试 血-组织-淋巴液和大分子理论 传输，并将这些应用于肺的测量 经血管交换。我们将严格审查 我们当前理论的假设，并评估其在 复杂的肺血管损伤的解释和 旨在预防或逆转这些疾病的疗法的行动 受伤。我们将扩展这一理论，以便它能被用来 分析已使用 微创体外检测系统，旨在 模拟该方法的潜在临床应用，以及 通过与淋巴数据的直接比较，验证了方法的有效性。 计算的输运系数归一化的各种方法 从外部检测到的跨血管蛋白流量将是 与肺干重正常化比较。我们建议 考察溶质和膜电性的重要性 流体和溶质跨毛细管运动的电荷。我们 将寻求对我们目前的 蛋白质分析的方法，以便我们可以增加数量 分析不同的蛋白质，并提高每种蛋白质的精确度 测量。此外，我们还将测量间质体积， 不含蛋白质的体积，以及肺淋巴中 通过插管的淋巴管，使我们能够研究 这些因素如何影响流体和流体的动力学 大分子交换。