BIOLOGICAL HAZARDOUS WASTE MANAGEMENT

这是一项研究一种创新和新颖的生物降解的建议 可适用于难降解化学品或复杂化学品的系统 各种化学品的混合物。该系统以胞外酶为基础， 催化自由基反应，降解木质素，性质 最顽固的化学物质。木质素是一种不溶的，非立体选择性的， 复杂的杂多聚合物。我们认为一种进化到退化的系统 木质素应该对许多种类的 顽固的人造化学物质。最终目标是减少 环境污染物的数量和毒性。系统应该是 适用于水和固体基质，其可用性可能是 这种化学物质的含量可能不像其他系统那样关键。另外， 如果这些系统是基于 木质纤维素营养物质。我们建议使用一种白腐菌来 从生物上降低与环境污染物相关的风险 危险废物场地或危险废物流。它是一种高度 集成的跨学科计划，其中各个领域的专业知识 在所有人都感兴趣的关键主题上发挥作用 个人调查员。该计划将与其他组织协调 测试TNT和PCP生物修复系统的项目 受污染的土壤和HMX污染的水。该计划将是 并支持环境中心的毒理学学位计划 毒物学。预计有几个学生将寻求联合 毒理学/分子生物学学位。该计划将涉及教职员工 犹他州化学与生物化学、生物学与环境工程 宾夕法尼亚州立大学分子和细胞生物学 圣母大学化学与生物化学专业。这项研究将 包括分子生物学、重组DNA技术、酶工程 酶学、生物降解研究、参与真菌竞争的因素 以及生物反应器设计和工程。

This is a proposal to investigate an innovative and novel biodegradation system that may be applicable to difficult-to-degrade chemicals or complex mixtures of chemicals. The system is based upon extracellular enzymes that catalyze free radical reactions that evolved to degrade lignin, natures most recalcitrant chemical. Lignin is an insoluble, non-stereoselective, complex heteropolymer. We propose that a system that evolved to degrade lignin should be useful for the biodegradation of a wide variety of very recalcitrant, man-made chemicals. The ultimate objective is to reduce the amount and toxicity of environmental pollutants. The system should be adapted to both aqueous and solid matrices and it may be that availability of the chemical may not be as critical as with other systems. Also, the fungus should have a major competitive advantage if the systems are based upon lignocellulose nutrients. We propose to use a white rot fungus to biologically reduce the risk associated with environmental pollutants in hazardous waste sites or hazardous waste streams. It is a highly integrated yet interdisciplinary program where various areas of expertise are brought to bear on critical subjects of interest to all of the individual investigators. The program will be coordinated with other projects to test the system for the bioremediation of TNT and PCP contaminated soil and HMX contaminated water. The program will be part of and support the Toxicology Degree Program of the Center for Environmental Toxicology. It is expected that several students will pursue a joint Toxicology/Molecular Biology degree. The program will involve faculty in Chemistry and Biochemistry, Biology and Environmental Engineering at Utah State University, and Molecular and Cell Biology at Pennsylvania State University and Chemistry and Biochemistry at Notre Dame. The research will include molecular biology, recombinant DNA technology, enzyme engineering, enzymology, biodegradation studies, factors involved in fungal competition and bioreactor design and engineering.