MOLECULAR GENETICS, CELL BIOLOGY AND BIOCHEMISTRY OF CANDIDA ALBICANS MALTASE

白色念珠菌麦芽糖酶的分子遗传学、细胞生物学和生物化学

• 批准号: 3790887
• 负责人: J BENNETT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790887
• 关键词: Candida albicans; alpha glucosidase; carbohydrate metabolism; enzyme activity; fungal genetics; intracellular transport; maltose; molecular cloning; permease; structural genes; sucrose; virulence; zymogens

Metabolism of sucrose and maltose by C. albicans was found to occur by intracellular transport and hydrolysis by maltase, an alpha-glucosidase. Maltase is produced as a less active proenzyme which is cleaved at the C terminal end to produce a more active enzyme. Maltase is coded for by the structural gene CAMAL2, which has been cloned from both cDNA and genomic DNA. Disruption of CAMAL2 is in progress in order to determine the function of CAMAL2 in virulence and saccharide metabolism. Transcription of CAMAL2 has been found to be glucose repressible. We have determined the upstream nucleotide sequences and will identify activation sites by several strategies. We have also demonstrated existence of a sucrose/maltose permease and are cloning the gene.

白念珠菌对蔗糖和麦芽糖的代谢是通过 麦芽糖酶是一种α-葡萄糖苷酶，可在细胞内转运和水解。 麦芽糖酶是以一种活性较低的前酶的形式产生的，它在C 末端产生更有活力的酶。麦芽糖酶是由 结构基因CAMAL2，已从两个基因中克隆 基因组DNA。CAMAL2的干扰正在进行中，以便确定 CAMAL2在毒力和糖代谢中的作用。 已发现CAMAL2的转录是葡萄糖可抑制的。我们 已经确定了上游核苷酸序列，并将识别 通过几种策略激活站点。我们还展示了 存在蔗糖/麦芽糖渗透酶，并正在克隆该基因。