课题基金 / 基金详情

CORE--SHARED LABORATORY CORE

CORE--SHARED LABORATORY CORE
核心--共享实验室核心
批准号:
3793635
负责人:
FRANK W FITCH
金额:
$0.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至

项目摘要

项目成果

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中文摘要
翻译
该核心支持所有六个实验室共同的活动 最有效和最经济的是, 需要高质量控制的业务。 核心还支持 核心运营以及常见设备的维护 六个实验室的研究人员共同使用的设备。 我们有 确定了这些操作通常会单独进行, 每个单独的实验室,使用类似的程序,并要求质量 控制 将这些业务合并为单一业务, 操作更有效,因此更经济,此外,它 提供了一个通用的标准,并节省了宝贵的时间和精力, 各实验室的技术人员。 服务和操作 核心小组提供的信息将包括: 使用单克隆抗体,无论是从腹水或肿块中获得 由使用哺乳动物细胞培养系统(Endotronics)的培养物产生 Acusyst-R),制备和测试用于流动的荧光探针 细胞计数分析、肿瘤和肿瘤的移植和维持 细胞系,转基因和SCID育种记录的维护 小鼠、培养基的制备和洗碗。 经验丰富的研究 技术人员将负责核心的日常操作 并将监督初级实验室助理, 用于洗碗和更日常的任务。
英文摘要
This core supports activities common to all six laboratories which are most efficiently and economically conducted as single operations that require high quality control. The core also supports maintenance of equipment used in the core operations as well as common equipment jointly used by investigators in the six laboratories. We have identified those operations which would ordinarily be done separately in each separate laboratory, using similar procedures and requiring quality control. Consolidation of these into single operations makes each operation more efficient and therefore economical, and in addition, it provides a common standard and saves valuable time and effort for the technical staff of the separate laboratories. Services and operations provided by the core will include: preparation of large pools of commonly used monoclonal antibodies whether obtained from ascitic fluid or mass produced from culture using a mammalian cell culture system (Endotronics Acusyst-R), preparing and testing fluorescent probes used for flow cytometric analyses, transplantation and maintenance of tumors and tumor cell lines, maintenance of breeding records for transgenic and SCID mice,.preparation of media, and dishwashing. An experienced research technician will be responsible for the day to day operations of the core and will supervise the junior laboratory helper who will be responsible for dishwashing and for the more routine tasks.
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LYMPHOID PROLIFERATION AND LIPOPROTEIN INTERACTIONS
  • 批准号:
    3920497
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    FRANK W FITCH
  • 依托单位:
CORE--ANALYTICAL REAGENTS FACILITY
  • 批准号:
    5205415
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    FRANK W FITCH
  • 依托单位:
    --
ANERGY AND SIGNALLING IN MURINE T CELL SUBSETS
  • 批准号:
    3727647
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    FRANK W FITCH
  • 依托单位:
ANERGY AND SIGNALLING IN MURINE T CELL SUBSETS
  • 批准号:
    3769871
  • 项目类别:
  • 资助金额:
    $0.0万
  • 财政年份:
    --
  • 负责人:
    FRANK W FITCH
  • 依托单位:
海外基金