STABILIZATION OF POLIOVIRUS INFECTIVITY TO LYOPHILIZATION

口服脊髓灰质炎病毒疫苗（OPV）在不使用时会迅速失去效力。 保持在冷冻状态。 OPV稳定至较低严格性 储存条件将极大地促进脊髓灰质炎病毒免疫接种， 程序. 一些疫苗可以冻干并以固态储存， 但冻干后脊髓灰质炎病毒的感染性大大降低。 我们系统地评估了不同的条件和添加剂， 寻找一种程序，使成功的冻干 脊髓灰质炎病毒。 冻干材料通过Elisa测定法 通过噬斑试验检测D抗原和感染性病毒。 戏剧性 通过使用脂质， 的试剂，使病毒存活率提高了1000， 20-40%的病毒感染性在冻干中存活。 尝试 继续进一步提高病毒对冻干的稳定性， 评价冻干制剂的时间和温度稳定性 病毒

Oral poliovirus vaccine (OPV) can rapidly lose potency when not maintained in a frozen state. Stabilization of OPV to less stringent storage conditions would greatly facilitate the poliovirus immunization program. Some vaccines can be lyophilized and stored in a solid state, but poliovirus infectivity is greatly diminished after lyophilization. We have systematically evaluated different conditions and additives in search of a procedure that would allow the successful lyophilization of poliovirus. Lyophilized material was assayed by both the Elisa assay for D antigen and for infectious virus by a plaque assay. Dramative improvements in viral survival were obtained with the use of a lipid based reagent, which increased viral survival by 1000 and resulted in 20-40% of the viral infectivity surviving lyophilization. Attempts are continuing to further increase virus stability to lyophilization and to evaluate the temporal and temperature stability of the lyophilized virus.