STABILIZATION OF POLIOVIRUS INFECTIVITY TO LYOPHILIZATION

冻干脊髓灰质炎病毒感染性的稳定

• 批准号: 3792549
• 负责人: R E LUNDQUIST
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792549
• 关键词: active immunization; enzyme linked immunosorbent assay; lyophilization; oral administration; pediatric pharmacology; plaque assay; poliomyelitis vaccine; poliovirus; reagent /indicator; virulence; virus antigen

Oral poliovirus vaccine (OPV) can rapidly lose potency when not maintained in a frozen state. Stabilization of OPV to less stringent storage conditions would greatly facilitate the poliovirus immunization program. Some vaccines can be lyophilized and stored in a solid state, but poliovirus infectivity is greatly diminished after lyophilization. We have systematically evaluated different conditions and additives in search of a procedure that would allow the successful lyophilization of poliovirus. Lyophilized material was assayed by both the Elisa assay for D antigen and for infectious virus by a plaque assay. Dramative improvements in viral survival were obtained with the use of a lipid based reagent, which increased viral survival by 1000 and resulted in 20-40% of the viral infectivity surviving lyophilization. Attempts are continuing to further increase virus stability to lyophilization and to evaluate the temporal and temperature stability of the lyophilized virus.

口服脊髓灰质炎病毒疫苗（OPV）在不使用时会迅速失去效力。 保持在冷冻状态。 OPV稳定至较低严格性 储存条件将极大地促进脊髓灰质炎病毒免疫接种， 程序. 一些疫苗可以冻干并以固态储存， 但冻干后脊髓灰质炎病毒的感染性大大降低。 我们系统地评估了不同的条件和添加剂， 寻找一种程序，使成功的冻干 脊髓灰质炎病毒。 冻干材料通过Elisa测定法 通过噬斑试验检测D抗原和感染性病毒。 戏剧性 通过使用脂质， 的试剂，使病毒存活率提高了1000， 20-40%的病毒感染性在冻干中存活。 尝试 继续进一步提高病毒对冻干的稳定性， 评价冻干制剂的时间和温度稳定性 病毒