STABILIZATION OF ORAL POLIOVIRUS VACCINE INFECTIVITY TO LYOPHILIZATION

口服脊髓灰质炎病毒疫苗(OPV)迅速失去传染性和效力，除非 以冷冻状态储存。这种疫苗投放量将大大提高 通过制定不那么严格的OPV储存条件。五花八门 世界各地的实验室都在试图稳定疫苗 通过添加稳定化合物或开发 成功的冷冻干燥程序。 我们试图开发一种脊髓灰质炎病毒的冷冻干燥程序。在……里面 与一些病毒疫苗相比，脊髓灰质炎病毒的传染性很强。 冷冻干燥后变淡。我们评价了不同的冷冻干燥方法 在添加各种物质之前的条件 冷冻干燥试图设计一种治疗脊髓灰质炎病毒的成功方法 病毒的稳定性通过D抗原和斑块的酶联免疫吸附试验来评估 传染性的化验。极大地包含了一种基于脂类的试剂 将病毒存活率提高10/3，导致20%-40%的存活率 冷冻干燥后的传染性。 进一步的研究将传染性病毒的恢复提高到更高的水平。 然而，50%的温度稳定性研究表明 冻干病毒与溶液中的病毒无显著差异。 我们目前的工作重点是提高材料的温度稳定性。 通过使用稳定衣壳的程序使病毒冻干 在不损害病毒粒子的有效性的情况下 疫苗。 将口服脊髓灰质炎疫苗成功稳定在较宽松的存储条件下将 促进疫苗分发，特别是在欠发达地区；以及 推动全球消灭脊髓灰质炎。

Oral poliovirus vaccine (OPV) rapidly loses infectivity and potency unless stored in a frozen state. Delivery of this vaccine would be greatly enhance by the development of less stringent OPV storage conditions. Various laboratories throughout the world are attempting to stabilize the vaccine either by the addition of stabilizing compounds or the development of successful lyophilization procedures. We have sought to develop a lyophilization procedure for poliovirus. In contrast to some viral vaccines, poliovirus infectivity is severely diminished after lyophilization. We have evaluated different lyophilizatio conditions in conjunction with the addition of various substances prior to lyophilization in an attempt to devise a successful approach for poliovirus Viral stability is assessed by an ELISA test for D-antigen and a plaque assay for infectivity. Inclusion of a lipid based reagent dramatically increases virus survival, by a factor of 10/3, resulting in 20-40% survival of infectivity after lyophilization. Further studies have raised the recovery of infectious virus to greater tha 50% However, temperature stability studies have indicated that the lyophilized virus is not significantly different than virus in solution. Our current efforts are focused on improving the temperature stability of the lyophilized virus by using procedures that stabilize the capsid architecture of the virion without impairing the effectiveness of the vaccine. Successful stabilization of OPV to less stringent storage conditions would facilitate vaccine distribution, particularly in less developed areas, and promote the global eradication of poliomyelitis.