CELLULAR AND HUMORAL IMMUNE RESPONSES TO RHINOVIRUSES

人鼻病毒免疫豚鼠的抗血清 (HRV)血清型被用来确定免疫印迹的反应性 HRV血清型衣壳表面多肽(VP1、VP2、VP3)系统 14(HRV14)，这是从HRV2开始的研究的继续。HRV14和HRV2 经CsCl2梯度洗脱，分离得到衣壳多肽 通过聚丙烯酰胺和转移到硝化棉的电泳法 用于免疫印迹的膜。血清中的免疫球蛋白结合用 免疫过氧化物酶方法学。仅针对HRV14的抗血清 中和HRV14，仅HRV2抗血清中和HRV2。 然而，针对53个个体血清型的大多数抗血清 与HRV2和HRV14反应。9份抗血清与两种抗体均无反应。 HRV和5个抗血清均与两种抗体反应。53人中的30人 抗血清仅与HRV2反应，19抗血清仅与HRV14反应。这个 反应性的模式与定义的光谱最相关 HRV血清型对抗病毒药物的敏感性 通过结合相对保守的鼻病毒衣壳来稳定鼻病毒衣壳 VP1衣壳表面有一个折叠的氨基酸序列。为 HRV14和HRV2，鉴定的抗体都是定向的 主要针对VP1的抗原决定簇，并且仅 不经常对抗VP2或VP3。数据表明，免疫原性 在HRV的进化过程中，抗原决定簇是保守的。这个 与衣壳反应血清型的鉴定 HRV14和HRV2都有或都没有HRV的多肽表明 已确定的抗原决定簇可能在HRV中共存，代表 HRV14和HRV2之间的转换，以及与之无关的抗原 可能代表着进一步的进化。

Antisera from guinea pigs immunized against individual human rhinovirus (HRV) serotypes were used to determine reactivity in an immunoblot system with the capsid surface peptides (VP1, VP2, VP3) of HRV serotype 14 (HRV14) in a continuation of studies begun with HRV2. HRV14 and HRV2 were purified by CsCl gradient, separated into capsid peptides by electrophoresis through polyacrylamide and transferred to nitrocellulose membranes for immunoblotting. Serum IgG binding was identified by immunoperoxidase methodology. Only the antiserum specific for HRV14 neutralized HRV14 and only the antiserum for HRV2 neutralized HRV2. However, the majority of the antisera against 53 individual serotypes reacted with HRV2 and HRV14. Nine of the antisera reacted with neither HRV and five of the antisera reacted with both. Thirty of the 53 antisera reacted only with HRV2 and 19 reacted only with HRV14. The pattern of reactivity was best correlated with a defined spectrum of susceptibility of the HRV serotypes to antiviral chemicals which stabilize the rhinovirus capsid by binding to a relatively conserved amino acid sequence occupying a fold in the capsid surface of VP1. For both HRV 14 and HRV2, the antibodies identified were directed predominantly against antigenic determinants of VP1, and only infrequently against VP2 or VP3. The data suggest that immunogenic antigenic determinants have been conserved in the evolution of HRV. The identification of antisera for serotypes which react with capsid peptides of both HRV14 and HRV2 or with neither HRV suggests that the antigenic determinants identified may coexist in HRV representing transitions between HRV14 and HRV2, and that unrelated antigenic representing further evolution may be present.