CELLULAR AND HUMORAL IMMUNE RESPONSES TO RHINOVIRUSES

先前的免疫印迹研究已经证明了针对VP 1的IgG 人鼻病毒血清型2（HRV 2）在所有成年人的血清测试，但 中和抗体和免疫印迹之间不存在相关性 结合抗体为了进一步研究这种相互作用， 对美国和智利的儿科患者进行了检查， 来自豚鼠的单特异性中和抗体进行了测试， 免疫印迹系统研究成人血清中的抗体同种型， 亚型HRV 2在WISH人羊膜细胞中复制，在CsCl上纯化 梯度，分离成主要的单个结构衣壳蛋白 (VP1、VP 2和VP 3），和 制备转移印迹到硝酸纤维素上的用于免疫印迹。除了少数 例外情况（1/23来自美国，4/79来自智利） 血清与VP 1反应。一些血清也有抗VP 2和VP 3的抗体， 比成人血清中观察到的百分比小。单特异性豚鼠 针对多种HRV的中和抗血清显示交叉反应性， 在免疫印迹系统中结合HRV 2的VP 1。免疫豚鼠 血清减少但不能完全消除人血清与VP 1的结合。 豚鼠抗血清不与VP 2或VP 3结合，也不与VP 2或VP 3竞争。 人IgG与VP 2和VP 3的结合。成人血清显示浓度 IgG、伊加和IgM的抗体与VP 1的依赖性结合。的IgG 抗体主要是IgG 1和IgG 3，几乎没有或没有IgG 2或IgG 4。 这些数据表明，存在与HRV肽结合的抗体， 从童年开始广泛，但肽的浓度和数量增加 随着年龄的增长而认识。豚鼠血清的交叉反应性 表明许多HRV共享表位，这些表位可以作为 另一种分组系统。人与几内亚的不完全竞争 猪血清提示识别一些相同和一些不相关的表位 在病毒衣壳上。

Previous immunoblotting studies have demonstrated IgG directed against VPl of human rhinovirus serotype 2 (HRV2) in the sera of all adults tested, but no correlation exists between neutralizing antibodies and immunoblot binding antibodies. In order to further study the interaction, sera from pediatric patients in the United States and Chile were examined, and sera from guinea pigs with monospecific neutralizing antibodies were tested in the immunoblot system. Adult sera were studied for the antibody isotype and subtype. HRV2 replicated in WISH human amnion cells, purified on CsC1 gradients, separated into the major individual structural capsid proteins (VP1, VP2, and VP3) by electrophoresis on polyacrylamide gels, and transblotted onto nitrocellulose was prepared for immunoblots. With few exceptions (1/23 from the U.S. and 4/79 from Chile) the tested pediatric sera reacted with VP1. Some sera also had antibody to VP2 and VP3 but a smaller percentage than noted with adult sera. Monospecific guinea pig neutralizing antisera against a variety of HRVs indicated cross reactive binding against VPl of HRV2 in the immunoblot system. Immune guinea pig sera reduced but did not completely eliminate binding of human sera to VP1. Guinea pig antisera did not bind to VP2 or VP3, and did not compete for binding of human IgG to VP2 and VP3. Adult sera demonstrated concentration dependent binding of antibodies to VPl for IgG, IgA, and IgM. The IgG antibodies were predominantly IgGl and IgG3 with little or no IgG2 or IgG4. The data suggest that antibodies which bind to HRV peptides are present widely from childhood but increase in concentration and number of peptides recognized as age increases. The cross reactivity of guinea pig sera suggests that many HRVs share epitopes that could serve as the basis for another system of grouping. The incomplete competition of human and guinea pig sera suggests recognition of some identical and some unrelated epitopes on the viral capsid.