EXPRESSION AND CHARACTERIZATION OF RECOMBINANT HUMAN INTERFERON ALPHA RECEPTOR

重组人干扰素α受体的表达和表征

• 批准号: 3804747
• 负责人: MARK P HAYES
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804747
• 关键词: binding proteins; complementary DNA; cytokine receptors; genetic manipulation; human genetic material tag; human tissue; interferons; monoclonal antibody; protein structure function; receptor binding; receptor coupling; receptor expression

The long-term goal of this study, initiated in June, 1991, is to express and characterize the protein encoded by the recently isolated cDNA for the putative Type I interferon receptor. Currently, very little is known about the biochemical and functional nature of the receptor protein. The plasmid containing the cDNA has been obtained from American Type Culture Collection. Initial efforts will be directed toward engineering the cDNA for optimal expression in different systems, both bacterial and mammalian. In addition, efforts to generate a soluble form of the receptor protein are underway. The protein products will be purified and used to generate both polyclonal and monoclonal antibodies to the protein. In addition, experiments will be performed to assess the capacity of this protein to bind various species of human interferon ` readily available in our laboratory. These studies should lead to an enhanced understanding of ligand-receptor interactions in the interferon system.

这项研究于1991年6月开始，其长期目标是表达 并表征由最近分离的cDNA编码的蛋白质， I型干扰素受体。 目前，我们对 关于受体蛋白的生化和功能性质。 的 含有cDNA的质粒已从美国典型培养物获得 收藏. 最初的努力将针对工程的cDNA 用于在细菌和哺乳动物的不同系统中的最佳表达。 此外，产生可溶形式的受体蛋白的努力 正在进行中 蛋白质产物将被纯化并用于产生 蛋白质的多克隆和单克隆抗体。 此外，本发明还提供了一种方法， 将进行实验以评估这种蛋白质的能力， 结合各种人干扰素， 实验室 这些研究应有助于增进对以下问题的了解： 干扰素系统中的配体-受体相互作用。