DEVELOPMENT AND CHARACTERIZATION OF A NEW RIPA FOR DETECTION OF HIV-2 ANTIBODIES

用于检测 HIV-2 抗体的新型 RIPA 的开发和表征

• 批准号: 3804863
• 负责人: R MAYNER
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804863
• 关键词: HIV envelope protein gp120; antiviral antibody; chimeric proteins; diagnosis quality /standard; epitope mapping; human immunodeficiency virus 2; immunoprecipitation; monoclonal antibody; radioimmunoassay; serum; synthetic peptide; western blottings

The sensitivity and specificity of commercial HIV-1 and HIV-2 antibody detection tests is difficult to measure. The sensitivity and specificity for a given test kit can vary from kit to kit and lot to lot and over time due to changes in the presence and concentration of detection antigens and the reagents used to produce the detection signal. In previous studies we have studied the markers of HIV-1 infection and the immune response for early and late antibody response (i.e. classification) to both linear and conformational epitomes of viral antigens. The markers of HIV-2 infection are less well defined due to the lack of specimens from early infection. The current study is designed to investigate whether the use of well defined antibody preparations can standardize kits by measuring test sensitivity and specificity on a kit by kit basis. Antibody preparations are being collected and analyzed by Western Blot, RIPA, synthetic peptide EIA, Recombinant EIA and all IFDA licensed kits. Also employed are Chimeric antibodies, that is a murine monoclonal antibody (Vh, VL gene) with human (Ch, Cl gene), to a gp120 epitope on ENV and two GAG epitopes, one located on p17 the other on p24. Currently, we are characterizing sera to be used in these studies.

商品化HIV-1和HIV-2抗体的敏感性和特异性 检测测试很难测量。 的敏感性和特异性 对于给定的检测试剂盒，试剂盒之间、批次之间以及随着时间的推移 由于检测抗原的存在和浓度的变化， 用于产生检测信号的试剂。 在以前的研究中， 研究了HIV-1感染的标志物和免疫反应， 早期和晚期抗体应答（即分类）， 病毒抗原的构象表位。 HIV-2感染的标志物 由于缺乏早期感染的标本， 目前的研究旨在调查是否使用井 确定的抗体制剂可通过测定试验使试剂盒标准化 灵敏度和特异性。 抗体制剂 通过Western Blot、RIPA、合成肽 EIA、重组EIA和所有IFDA许可试剂盒。 还雇用了 嵌合抗体，即鼠单克隆抗体（Vh、VL基因） 与人（Ch，Cl基因），与ENV上的gp 120表位和两个GAG表位， 一个位于P17，另一个位于P24。 目前，我们正在鉴定血清 用于这些研究。