THE PROTECTIVITY OF AN ANTI-SUBTYPE D ANTIBODY AGAINST HBV INFECTION

抗 D 亚型抗体对 HBV 感染的保护作用

• 批准号: 3792647
• 负责人: M W YU
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH HEMATOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792647
• 关键词: Pan; antibody specificity; antiviral antibody; biomarker; hepatitis B; hepatitis B antigens; hepatitis B virus group; hepatitis vaccine; immunoglobulin G; immunoglobulin M; liver function; monoclonal antibody; neutralizing antibody

As described in the previous annual report, a monoclonal anti-d antibody is capable of neutralizing the infectivity of hepatitis B virus (HBV) of the adw subtype in a susceptible chimpanzee. We have since inoculated a control chimpanzee with a mixture of this monoclonal anti-d antibody and HBV of the ayw subtype (1000 CID50). The levels of HBV serological markers (Hbsag, anti-HBs and anti-HBc) and ALT liver enzyme were monitored with weekly blood specimens. Hbsag was detected beginning four weeks after inoculation, and the ALT level exceeded 2.5 times baseline at 13 weeks, peaking at 15 weeks. Thus, this monoclonal anti-d antibody is specific to ad but not to ay subtype and the epitopes recognized by this anti-d may be a potential HBV vaccine.

如前一份年度报告所述， 能够中和人的B型肝炎病毒（HBV）的感染性， adw亚型在易感的黑猩猩中。 从那以后，我们接种了 用这种单克隆抗-D抗体和 ayw亚型HBV（1000 CID 50）。 HBV血清学标志物水平 用ELISA法检测HBsAg、抗-HBs、抗-HBc和ALT肝酶， 每周血液样本。 HBsAg检测开始四周后， 接种，并且ALT水平在13周时超过基线的2.5倍， 在15周时达到峰值。 因此，这种单克隆抗-d抗体对 而不是Ay亚型，并且这种抗-D识别的表位可能是 一种潜在的乙肝疫苗