THE PROTECTIVITY OF AN ANTI-SUBTYPE D ANTIBODY AGAINST HBV INFECTION

如上一份年度报告所述，一种抗d的单抗是 能够中和乙肝病毒(乙肝病毒)的传染性 易感黑猩猩的ADW亚型。自那以后，我们接种了一种 用这种抗d单抗和黑猩猩的混合物控制黑猩猩 Ayw亚型的乙肝病毒(1000CID50)。乙肝病毒血清标志物水平 (乙肝表面抗原、抗-HBs、抗-HBc)和ALT肝酶 每周抽血一次。四周后开始检测到乙肝表面抗原 接种，13周时ALT水平超过基线的2.5倍， 在15周时达到顶峰。因此，这种抗d的单抗是特异的。 AD而不是Ay亚型，并且该抗-d识别的表位可能是 一种潜在的乙肝疫苗。

As described in the previous annual report, a monoclonal anti-d antibody is capable of neutralizing the infectivity of hepatitis B virus (HBV) of the adw subtype in a susceptible chimpanzee. We have since inoculated a control chimpanzee with a mixture of this monoclonal anti-d antibody and HBV of the ayw subtype (1000 CID50). The levels of HBV serological markers (Hbsag, anti-HBs and anti-HBc) and ALT liver enzyme were monitored with weekly blood specimens. Hbsag was detected beginning four weeks after inoculation, and the ALT level exceeded 2.5 times baseline at 13 weeks, peaking at 15 weeks. Thus, this monoclonal anti-d antibody is specific to ad but not to ay subtype and the epitopes recognized by this anti-d may be a potential HBV vaccine.