INTERACTIONS OF MODIFIED HEMOGLOBINS WITH IRON CHELATORS

在目前旨在降低氧化剂的氧化作用的策略中， HBOCs是使用铁螯合剂，以防止潜在的分子 和铁诱导的氧自由基引起的细胞损伤。 我们 研究了去铁胺和新的 开发吡喃酮（乙基麦芽酚）的自氧化和氢的速率 过氧化物介导的交联血红蛋白的氧化。 有一个 自氧化速率和半染色质含量均略有下降 当乙基麦芽酚被包括在 这些血红蛋白 相反，包含去铁胺导致 氧化产物的增加。 在反应混合物中，并且在 过量过氧化氢的存在下，去铁胺导致20 - 30% 高铁血红蛋白升高，同时血红素略有减少 阵 另一方面，乙基麦芽酚的作用很小或没有作用。 似乎与乙基麦芽酚不同，去铁胺独立，如果其 铁离子的螯合特性，作为氧化剂。 这可能与 直接与氧自由基相互作用， 增加高铁血红蛋白的形成 计划开展进一步研究， 验证这一点，并研究潜在的分子机制， 去铁胺的作用。

Among the current strategies aimed at decreasing the oxidant effect of HBOCs is the use of iron chelators to prevent the potential molecular and cellular damage caused by iron induced oxygen free radicals. We studies the effects of interactions of desferrioxamine and newly developed pyrone (ethyl maltol) on the rate of autoxidation and hydrogen peroxide mediated oxidation of crosslinked hemoglobins. There was a slight decrease in both the rate of autoxidation and hemichrome formation when ethyl maltol was included in the incubation media of these hemoglobins. Conversely, inclusion of desferrioxamine led to an increase in oxidation products. In the reaction mixture, and in the presence of excess hydrogen peroxide, desferrioxamine led to a 20-30% rise in methemoglobin coupled with slight reduction in the hemichrome formation. Ethyl maltol, on the other hand, had little or no effect. It appears that unlike ethyl maltol, desferrioxamine independent if its iron cheating properties, acts as an oxidizing agent. This may relate to its ability to interact directly with oxygen free radicals resulting in increased methemoglobin formation. Further studies are planned to verify this and to investigate the underlying molecular mechanism of desferrioxamine's action.