HEMOGLOBIN-OXYGEN EQUILIBRIUM STUDIES

我们最近收购了Hemox Analyzer，它可以实现自动 全血和离体血氧平衡曲线的记录 血红蛋白。该系统将用于确定： 1)氧平衡曲线及若干参数的测定 重要的和相关的变量，如血红蛋白的协作性，Boher 效应、温度效应等多项化学改性 血红蛋白制剂。 2)成人镰状细胞标本的全血氧平衡曲线 接受重组人的联合治疗的患者 促红细胞生成素和羟基脲。胎儿血红蛋白升高的影响 氧结合的合成(EPO/Hu给药的结果) S的红细胞内血红蛋白的特性将被测定。 该项目的这一具体部分将在#年在我们的实验室进行。 与G·罗杰斯博士和细胞实验室的谢克特合作 生物学，NIDDK，Bld.10。

We have recently acquired The Hemox Analyzer which enables automatic recording of oxygen equilibrium curves of whole blood and isolated hemoglobins. This system will be utilized in determining: 1) Oxygen equilibrium curves as well as the determination of a number of important and relevant variables such as hemoglobin cooperativity, Boher effect, temperature effect, etc. for a number of chemically modified hemoglobin preparations. 2) Whole blood oxygen equilibrium curves in samples from adult sickle cell patients undergoing treatment with a combination therapy of recombinant erythropoietin and hydroxyurea. The effect of increased fetal hemoglobin synthesis ( a result of EPO/HU administration) on oxygen binding characteristics of the intraerythrocytic hemoglobin S will be determined. This specific part of the project will be carried out here at our lab in collaboration with Drs. G. Rodgers and A Schechter from the Lab of Cell Biology, NIDDK, Bldg. 10.