GENETIC DETERMINATIONS OF NEUROVIRULENCE IN HIV-1

HIV-1 神经毒力的遗传决定

• 批准号: 3811268
• 负责人: R ANAND
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811268
• 关键词: AIDS; AIDS dementia complex; antibody specificity; gene duplication; human immunodeficiency virus 1; human tissue; immunoprecipitation; molecular cloning; molecular genetics; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; radioimmunoassay; virus cytopathogenic effect; virus morphology; virus protein

HIV-1,br was isolated from the autopsied brain tissue of a 57-year-old man died of progressive dementing illness. This virus was shown to be HIV-1 by hybridization to HIV-specific DNA probes. The expression of viral proteins as tested by radioimmunoprecipitation assay revealed the presence of HIV-1 specific proteins. HIV-1,br replicated in cultures of CD4+ T-lymphoid cells and induced cytopathic effects in these cells. HIV-1,br also replicated in monocytoid cell lines. The genetic nature of this isolate was determined by molecular cloning and sequencing of the 3'-half of the genome. DNA sequence information established that HIV-l,br is unique HIV-1 isolate. A stretch of approximately 30 bases in the nef gene of HIV-l,br was found duplicated when compared with the other sequenced HIV-1 genomes (Virology, 1989;168:79-89). To determine the functional significance of nef duplication in neuropathogenesis, we have generated primers flanking the duplication. By using these primers we are examining a large number of neural and non-neural isolates of HIV-1 to explore the prevalence of this duplication.

HIV-1,br 从一名 57 岁男性尸检脑组织中分离出来 死于进行性痴呆症。该病毒被证明是 HIV-1 与 HIV 特异性 DNA 探针杂交。病毒蛋白的表达 放射免疫沉淀试验显示 HIV-1 的存在 特定蛋白质。 HIV-1,br 在 CD4+ T 淋巴细胞培养物中复制 并在这些细胞中诱导细胞病变效应。 HIV-1,br 也在 单核细胞系。该分离株的遗传性质由以下因素决定： 基因组 3'-半部分的分子克隆和测序。 DNA序列 信息表明 HIV-1,br 是独特的 HIV-1 分离株。一段 HIV-l,br 的 nef 基因中大约有 30 个碱基重复 与其他已测序的 HIV-1 基因组（病毒学、 1989；168：79-89）。确定 nef 的功能意义 神经发病机制中的重复，我们生成了侧翼的引物 复制。通过使用这些引物，我们正在检查大量 HIV-1 的神经和非神经分离株，以探讨该病毒的流行情况 复制。