HIV IN PATIENTS AND ANIMALS--CELL MEDIATED IMMUNITY AND VIRAL ISOLATION

病毒分离研究从上一财年的样本继续进行 取自参与NIAID-AIDS治疗试验的患者。有耐心的 外周血淋巴细胞(PBL)最初与正常人共培养 PBL爆炸。用逆转录酶试验检测病毒，并 杜邦p24抗原检测系统。人们的注意力集中在适应 为了组织培养T细胞系，从其中5个连续分离出来 4例患者均超过4年。目前正在进行的工作是 分析这些分离株随时间的变异与其 抗体中和和DNA序列模式，试图 将这些变化与艾滋病临床分期的差异联系起来。 类似的研究主要集中在从PBL中分离和鉴定病毒 取自智利的艾滋病患者。我们发现，许多分离株作为 以聚合酶链式反应和抗体为特征 中和交叉反应类似于IIIB分离物，而 其他人则不同。进一步的定性将是必要的，以了解 它们与世界各地其他常见的分离株不同。 与JHMC启动了一项合作研究，以进行序贯病毒 艾滋病患者骨髓前后PBL的分离 移植。到目前为止，已有5名患者接受了隔离 每周做一次骨髓和外周血淋巴细胞检查。结果是可变的，但 移植后一般都呈现病毒减少的趋势。 PBL与淋巴结共培养的后续病毒分离尝试 1987年用HIV-1免疫恒河猴的结果仍然是阴性的 即使在5-氮胞苷诱导后也是如此。目前还没有临床上 这些动物的变化，然而，2仍然是血清阳性和所有4显示 聚合酶链法分析表明含有病毒DNA的迹象 反应技术。

Virus isolation studies were continued from the previous FY with samples taken from patients involved with the NIAID-AIDS treatment trials. Patient peripheral blood lymphocytes (PBL) were initially cocultured with normal PBL blasts. Virus was detected using the reverse transcriptase assay and the Dupont p24 antigen detection system. Attention was focused on adapting to tissue culture T cell lines, sequential isolates from 5 of these patients obtained over 4-year period. Work is currently in progress to analyze the variation in these isolates over tim as related to their antibody neutralization and DNA sequence patterns, in an attempt to correlate these changes with differences in clinical stages of AIDS. Similar studies focused on isolating and characterizing virus from PBL taken from AIDS patients in Chile. We found that many of the isolates as characterized by t polymerase chain reaction (PCR) and antibody neutralization crossreactivity were similar to the IIIB isolate while others were different. Further characterization will be necessary to see if they vary from other common isolates worldwide. A collaborative study was initiated with JHMC to do sequential virus isolations on PBL from AIDS patients before and after bone marrow transplantation. 5 patients have been followed to date with isolations performed on bone marrow and PBL every weeks. Results are variable, but generally have shown a virus reduction trend after transplantation. Follow-up virus isolation attempts on cocultivation of PBL and lymph nodes from rhesus monkeys immunized with HIV-1 in 1987 continue to be negative even after induction with 5-azacytidine. There have been no clinical changes in these animals, however 2 are still seropositive and all 4 show indications of harboring viral DNA when analyzed by the polymerase chain reaction technique.