HIV IN PATIENTS AND ANIMALS--CELL MEDIATED IMMUNITY AND VIRAL ISOLATION

病毒分离研究从上一财政年度继续进行， 来自参与NIAID-AIDS治疗试验的患者。患者 外周血淋巴细胞（PBL）最初与正常人共培养， PBL胚细胞。使用逆转录酶测定法检测病毒， 杜邦p24抗原检测系统。注意力集中在适应 组织培养T细胞系，从其中5个连续分离 4年以上的患者。目前正在开展工作， 分析这些分离株随时间的变化， 抗体中和和DNA序列模式，试图 将这些变化与艾滋病临床分期的差异联系起来。 类似的研究集中在从PBL中分离和表征病毒 来自智利的艾滋病患者。我们发现，许多分离物， 以聚合酶链反应（PCR）和抗体为特征， 中和交叉反应性与IIIB分离物相似， 其他人则不同。需要进一步表征，以确定 它们不同于世界上其他常见的分离株。 与JHMC开展了一项合作研究， 艾滋病患者骨髓前后外周血淋巴细胞的分离 移植迄今为止，已对5例患者进行了隔离随访 每周进行骨髓和PBL检查。结果是可变的，但 在移植后一般都表现出病毒减少的趋势。 PBL和淋巴结共培养的后续病毒分离尝试 1987年用HIV-1免疫的恒河猴， 即使在用5-氮杂胞苷诱导后。尚无临床 这些动物中的变化，然而，2只仍为血清阳性，所有4只均显示 当通过聚合酶链分析时，存在病毒DNA的迹象 反应技术

Virus isolation studies were continued from the previous FY with samples taken from patients involved with the NIAID-AIDS treatment trials. Patient peripheral blood lymphocytes (PBL) were initially cocultured with normal PBL blasts. Virus was detected using the reverse transcriptase assay and the Dupont p24 antigen detection system. Attention was focused on adapting to tissue culture T cell lines, sequential isolates from 5 of these patients obtained over 4-year period. Work is currently in progress to analyze the variation in these isolates over tim as related to their antibody neutralization and DNA sequence patterns, in an attempt to correlate these changes with differences in clinical stages of AIDS. Similar studies focused on isolating and characterizing virus from PBL taken from AIDS patients in Chile. We found that many of the isolates as characterized by t polymerase chain reaction (PCR) and antibody neutralization crossreactivity were similar to the IIIB isolate while others were different. Further characterization will be necessary to see if they vary from other common isolates worldwide. A collaborative study was initiated with JHMC to do sequential virus isolations on PBL from AIDS patients before and after bone marrow transplantation. 5 patients have been followed to date with isolations performed on bone marrow and PBL every weeks. Results are variable, but generally have shown a virus reduction trend after transplantation. Follow-up virus isolation attempts on cocultivation of PBL and lymph nodes from rhesus monkeys immunized with HIV-1 in 1987 continue to be negative even after induction with 5-azacytidine. There have been no clinical changes in these animals, however 2 are still seropositive and all 4 show indications of harboring viral DNA when analyzed by the polymerase chain reaction technique.