MARROW GRAFT FAILURE IN ALLOGENIC BONE MARROW TRANSPLANTATION

大量的观察表明，宿主淋巴细胞，特别是 细胞毒性T细胞（CTL）可能在介导同种异体免疫应答中起重要作用。 骨髓移植排斥在小鼠模型系统中，从小鼠中克隆CTL。 亚致死剂量照射动物的脾脏， 骨髓移植发现克隆的CTL足以影响 致死剂量照射后去T细胞同种异体骨髓的排斥反应 动物CTL对骨髓移植物的排斥反应是对MHC特异性的 基因产物由骨髓细胞表达，并与 单个克隆的细胞毒性特异性。由于CTL可以 排斥骨髓移植，抗CD3单克隆抗体的管理， 已经研究了体内作为增强T细胞耗尽的移植的手段， 亚致死剂量照射小鼠宿主的骨髓。此外，细胞能够 特异性抑制前体CTL的研究已经在体外和体内进行， vivo.为了类似地评估人CTL，异种特异性人CTL被称为人CTL。 已经研究了细胞毒性反应。异种抗原良好的特异性 人类T细胞的识别表明，在异种移植中， 组织，如在MHC不匹配的同种异体组织的移植中， 特定的组织抗原，因此组织分型，可能是相关的 考虑.已经表征了人CTL的产生， 在这一代人中，某些必要的细胞相互作用 鉴定

A number of observations have suggested that host lymphocytes, specifically cytotoxic T cells (CTL) may play a significant role in mediating allogeneic marrow graft rejection. In a murine model system, CTL were cloned from the spleens of sublethally irradiated animals which had rejected MHC disparate marrow grafts. It was found that cloned CTL were sufficient to effect rejection of T cell depleted allogeneic marrow in lethally irradiated animals. The rejection of marrow grafts by CTL was specific for the MHC gene products expressed by the marrow cells and correlated with the cytotoxic specificity of the individual clones. Since CTL can therefore reject marrow grafts, the administration of anti-CD3 monoclonal antibody in vivo has been studied as a means to enhance engraftment of T cell depleted marrow in sublethally irradiated murine hosts. In addition, cells capable of specifically suppressing precursor CTL have been studied in vitro and in vivo. In order to similarly evaluate human CTL, xenospecific human cytotoxic responses have been studied. The fine specificity of xenoantigen recognition by human T cells suggests that in transplantation of xenogeneic tissue, as in transplantation of MHC mismatched allogeneic tissue, the particular tissue antigens, and therefore tissue typing, may be a relevant consideration. The generation of human CTL has been characterized and certain essential cellular interactions in that generation have been identified.