T CELL FUNCTION IN T CELL DEPLETED BONE MARROW TRANSPLANTATION

从骨髓中清除表达T细胞表面的细胞 在同种异体和自体骨髓中， 移植--作为预防移植物抗宿主病的一种手段， 同种异体骨髓移植，并作为消除或 从骨髓中清除表达T细胞表面标志物的恶性细胞 通过自体骨髓移植治疗T细胞肿瘤。 开发了用于消耗正常和恶性T细胞的方法， 通过使用淘洗和衍生的单克隆 对T细胞特有的细胞表面分子特异的抗体。 这些 方法用于制定临床方案，以评估 利用同种异体HLA错配、T细胞耗竭的可行性 同种异体骨髓和清除恶性T细胞的自体骨髓， 侵袭性血液淋巴细胞生成性恶性肿瘤的治疗。 恒河猴的临床前研究表明，功能性T细胞 在接受T细胞耗尽的自体移植的动物中产生群体 骨髓。 CD 4 + T细胞重建的时间过程和发展 体内T细胞免疫活性与T细胞数量相关 在骨髓中输注，与骨髓细胞剂量或 造血重建，增加了残留T细胞 输注的T细胞耗尽的骨髓中的细胞在 随后T细胞群的产生。 的机制 输注的成熟T细胞产生重建的外周T细胞 在鼠模型中研究了群体。 功能 这些再生T细胞群体的能力也是令人感兴趣的。 人辅助性T细胞对异种MHC编码抗原的应答 已经研究了通过刺激鼠细胞群表达的蛋白质， 发现在评估人T辅助细胞中具有特殊用途 功能在于，

The elimination from marrow of cells expressing T cell surface markers is of interest both in allogeneic and autologous marrow transplantation -- as a means of preventing graft versus host disease in allogeneic marrow transplantation and as a means of eliminating or purging malignant cells expressing T cell surface markers from marrow in treating T cell neoplasms by autologous marrow transplantation. Approaches were developed for depleting normal and malignant T cell marrow populations by using elutriation and deriving monoclonal antibodies specific for cell surface molecules unique to T cells. These approaches were used to develop clinical protocols to assess the feasibility of utilizing allogenic HLA-mismatched, T cell depleted allogeneic marrow and autologous marrow purged of malignant T cells in the treatment of aggressive hematolymphopoietic malignancies. Preclinical studies in rhesus monkeys demonstrated that functional T cell populations are generated in animals receiving T cell depleted autologous marrow. The time course of CD4+ T cell reconstitution and development of in vivo T cell immunocompetence correlated with the number of T cells infused in the marrow, and did not correlate with marrow cell dose or hematopoietic reconstitution, raising the possibility that residual T cells in the infused T cell depleted marrow played a central role in the generation of subsequent T cell populations. The mechanisms by which infused, mature T cells generate reconstituting peripheral T cell populations is under investigation in murine models. The functional capacities of these regenerated T cell populations is also of interest. The human T helper cell response to xenogenic MHC encoded antigens expressed by stimulating murine cell populations has been studied and found to be of special use in the assessment of human T helper cell function in that this