EXPRESSION OF VP3 & VP7 GENES OF ROTAVIRUS USING A BACULOVIRUS EXPRESSION VECTOR

VP3的表达

• 批准号: 3818299
• 负责人: K NISHIKAWA
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818299
• 关键词: Rotavirus; antibody neutralization test; complementary DNA; disease /disorder model; gene expression; genetic manipulation; molecular cloning; swine; transposon /insertion element; virus envelope; virus genetics; virus protein

The VP3 and VP7 outer capsid proteins of rotaviruses are major neutralization antigens and play an important role in the initiation of infection. In order to better understand the function and antigenicity of each protein, we constructed full length cDNA clones of these genes and inserted them into the polyhedrin gene of baculovirus. We plan to carry out the passive immunization studies in the piglet model using antiserum produced with such proteins expressed by baculovirus-rotavirus VP3 or VP7 recombinant.

轮状病毒的VP 3和VP 7外衣壳蛋白是主要的 中和抗原，并发挥重要作用， 感染的开始。 为了更好地理解 功能和抗原性，我们构建了完整的 这些基因的cDNA克隆，并将它们插入到 杆状病毒多角体蛋白基因。 我们计划进行被动的 使用产生的抗血清在仔猪模型中进行免疫研究 用杆状病毒-轮状病毒VP 3或VP 7表达的这种蛋白质 重组体