THE COMPLETE NUCLEOTIDE SEQUENCE OF DENGUE TYPE 4 VIRUS

登革4型病毒42SRNA(814669株)基因文库的构建 是建造的。多个重叠的DNA克隆代表 整个登革热基因组被用来确定完整的 核苷酸序列。基因组包含10,644个核苷酸，其中 编码3386多蛋白的单一开放阅读框 氨基酸。登革热病毒的同源性比对， 这三种结构蛋白命名为衣壳蛋白(S)， 膜(M)和包膜(E)位于N端， 病毒功能的NS1、NS2a等非结构蛋白 对宿主细胞的吸收、血凝和 与中和抗体的反应性。氨基和同源 分析表明，它们之间存在着密切的层序关系 在不同黄病毒的囊膜糖蛋白中。这个 登革热NS1非结构蛋白已被证明是可溶的 补体固定抗原。关于YF或登革热病毒的最新研究 已经证明NS1能诱导小鼠对其各自的免疫应答 病毒感染。NS1非结构蛋白在生物信息学中的应用 预防登革热的免疫接种特别吸引人，因为 包膜糖蛋白增强免疫的可能性- 可以避免特定的抗体。

A cDNA library from the dengue type 4 virus 42s RNA (strain 814669) was constructed. Multiple overlapping DNA clones which represent the entire dengue genome were used to determine the complete nucleotide sequence. The genome contains 10,644 nucleotides with a single open reading frame that encodes a polyprotein of 3386 amino acids. Homology alignment of the dengue type viruses, indicated that the three structural proteins designated capsid(s), membrane (M), and envelope (E), are located at the N-terminus and nonstructural proteins in the order of NS1, NS2a, viral functional activities such as absorption to host cells, hemagglutination and reactivity with neutralizing antibodies. Amino and homology analysis indicates that there is a close sequence relationship among the envelope glycoproteins of different flaviviruses. The dengue NS1 nonstructural protein has been shown to be the soluble complement fixing antigen. Recent studies with YF or dengue virus have shown that NS1 induces immunity in mice against its respective virus infection. The use of NS1 nonstructural protein for immunization against dengue is especially attractive because the possibility of immune enhancement due to envelope glycoprotein- specific antibodies could be avoided.