THE COMPLETE NUCLEOTIDE SEQUENCE OF DENGUE TYPE 4 VIRUS

登革热 4 型病毒的完整核苷酸序列

• 批准号: 3818250
• 负责人: C J LAI
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818250
• 关键词: antibody neutralization test; antiviral antibody; dengue; dengue virus; encephalitis virus; gene expression; genetic library; genetic translation; genome; glycoproteins; immunogenetics; laboratory mouse; nucleic acid sequence; protein biosynthesis; protein sequence; proteolysis; thrombospondins; viral vaccines; virus RNA; virus envelope; virus genetics; virus infection mechanism; virus protein

A cDNA library from the dengue type 4 virus 42s RNA (strain 814669) was constructed. Multiple overlapping DNA clones which represent the entire dengue genome were used to determine the complete nucleotide sequence. The genome contains 10,644 nucleotides with a single open reading frame that encodes a polyprotein of 3386 amino acids. Homology alignment of the dengue type viruses, indicated that the three structural proteins designated capsid(s), membrane (M), and envelope (E), are located at the N-terminus and nonstructural proteins in the order of NS1, NS2a, viral functional activities such as absorption to host cells, hemagglutination and reactivity with neutralizing antibodies. Amino and homology analysis indicates that there is a close sequence relationship among the envelope glycoproteins of different flaviviruses. The dengue NS1 nonstructural protein has been shown to be the soluble complement fixing antigen. Recent studies with YF or dengue virus have shown that NS1 induces immunity in mice against its respective virus infection. The use of NS1 nonstructural protein for immunization against dengue is especially attractive because the possibility of immune enhancement due to envelope glycoprotein- specific antibodies could be avoided.

登革4型病毒814669株42 s RNA cDNA文库的构建 构建了 多个重叠的DNA克隆， 使用整个登革热基因组来确定完整的 核苷酸序列。 基因组包含10，644个核苷酸， 编码3386多聚蛋白的单个开放阅读框 个氨基酸 登革型病毒的同源性比对， 表明这三种结构蛋白命名为衣壳， 膜（M）和包膜（E）位于N-末端， 非结构蛋白，按NS 1、NS 2a、病毒功能蛋白的顺序， 活性如对宿主细胞的吸收、血凝和 与中和抗体反应。 氨基酸和同源性 分析表明，它们之间存在密切的序列关系 在不同黄病毒的包膜糖蛋白中。 的 登革NS 1非结构蛋白已被证明是可溶性的， 补体结合抗原 最近对YF或登革热病毒的研究 已经表明，NS 1诱导小鼠对其各自的 病毒感染。 NS 1非结构蛋白用于 针对登革热的免疫接种特别有吸引力，因为 由于包膜糖蛋白的免疫增强的可能性- 可以避免特异性抗体。