THE COMPLETE NUCLEOTIDE SEQUENCE OF DENGUE TYPE 4 VIRUS

登革4型病毒814669株42 s RNA cDNA文库的构建 构建了 多个重叠的DNA克隆， 整个登革热基因组被用来确定完整的 核苷酸序列。 基因组包含10，644个核苷酸， 编码3386多聚蛋白的单个开放阅读框 个氨基酸 登革型病毒的同源性比对， 表明这三种结构蛋白命名为衣壳， 膜（M）和包膜（E）位于N-末端， 非结构蛋白，按NS1、NS2a、病毒功能蛋白的顺序， 活性如对宿主细胞的吸收、血凝和 与中和抗体反应。 氨基酸和同源性 分析表明，它们之间存在密切的序列关系 在不同黄病毒的包膜糖蛋白中。 的 登革NS1非结构蛋白已被证明是可溶性的， 补体结合抗原 最近对YF或登革热病毒的研究 已经表明，NS1诱导小鼠对其各自的 病毒感染。 NS1非结构蛋白用于 针对登革热的免疫接种特别有吸引力，因为 由于包膜糖蛋白的免疫增强的可能性- 可以避免特异性抗体。

A cDNA library from the dengue type 4 virus 42s RNA (strain 814669) was constructed. Multiple overlapping DNA clones which represent the entire dengue genome were used to determine the complete nucleotide sequence. The genome contains 10,644 nucleotides with a single open reading frame that encodes a polyprotein of 3386 amino acids. Homology alignment of the dengue type viruses, indicated that the three structural proteins designated capsid(s), membrane (M), and envelope (E), are located at the N-terminus and nonstructural proteins in the order of NS1, NS2a, viral functional activities such as absorption to host cells, hemagglutination and reactivity with neutralizing antibodies. Amino and homology analysis indicates that there is a close sequence relationship among the envelope glycoproteins of different flaviviruses. The dengue NS1 nonstructural protein has been shown to be the soluble complement fixing antigen. Recent studies with YF or dengue virus have shown that NS1 induces immunity in mice against its respective virus infection. The use of NS1 nonstructural protein for immunization against dengue is especially attractive because the possibility of immune enhancement due to envelope glycoprotein- specific antibodies could be avoided.