MOLECULAR CLONING AND ANALYSIS OF TUMOR-ASSOCIATED ANTIGENS

肿瘤相关抗原的分子克隆和分析

• 批准号: 3916369
• 负责人: J KANTOR
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916369
• 关键词: breast neoplasms; carcinoma; complementary DNA; gene expression; genetic library; interferons; molecular cloning; monoclonal antibody; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; nucleic acid hybridization; nucleic acid probes; plasmids; serum; tissue /cell culture; transfection; tumor antigens

Studies carried out in our lab as well as others have demonstrated the presence of the TAG-72 and CEA tumor associated antigens in a high percentage of human carcinomas. Our studies have focused on understanding the role of these molecules in tumorigenesis as well as understanding the molecular mechanisms involved in the regulation of expression of these antigens. Specific probes for members of the CEA gene family will be used to study their expression in various tissues and cell lines and to examine the mechanism of regulation of CEA expression by interferons as well as other biological response modifiers. In addition, the gene encoding CEA will be cloned and transfected into mouse metastatic cell lines in order to obtain a mouse model for the study of anti- tumor agents. Polyclonal antisera to deglycosylated TAG-72 and monoclonal antibodies to native TAG-72 will be used to obtain clones from cDNA libraries. Positive clones will be sequenced and used for analysis of TAG-72 expression in normal and tumor cell line and tissues.

在我们实验室和其他实验室进行的研究表明， TAG-72和CEA肿瘤相关抗原的存在， 高比例的人类癌症。 我们的研究集中在 了解这些分子在肿瘤发生中的作用 随着了解参与的分子机制， 调节这些抗原的表达。 特异性探针 CEA基因家族的成员将被用于研究他们的 在各种组织和细胞系中的表达，并检查 干扰素调控CEA表达的机制 作为其他生物反应调节剂。 此外，该基因 将编码CEA的基因克隆并转染到小鼠转移瘤中， 细胞系，以获得用于研究抗- 肿瘤因子 去糖基化TAG-72的多克隆抗血清和 天然TAG-72的抗体将用于从cDNA获得克隆 图书馆. 将对阳性克隆进行测序并用于分析 TAG-72在正常和肿瘤细胞系和组织中的表达。