MOLECULAR CLONING AND ANALYSIS OF TUMOR-ASSOCIATED ANTIGENS

在我们实验室和其他实验室进行的研究表明 TAG-72和CEA肿瘤相关抗原的存在 人类癌症的比例很高。我们的研究主要集中在 了解这些分子在肿瘤发生中的作用 随着对分子机制的理解涉及到 调节这些抗原的表达。特定的探测对象 CEA基因家族的成员将被用于研究他们的 在各种组织和细胞系中的表达，并检测 干扰素对CEA表达的调节机制 作为其他生物反应调节剂。此外，该基因 CEA编码基因将被克隆并转染到小鼠转移瘤中 为了获得小鼠模型细胞株进行抗肿瘤作用的研究 肿瘤制剂。 脱糖化TAG-72多克隆抗血清及单抗 针对天然TAG-72的抗体将用于从cDNA中获得克隆 图书馆。阳性克隆将被测序并用于分析 TAG-72在正常和肿瘤细胞系及组织中的表达。

Studies carried out in our lab as well as others have demonstrated the presence of the TAG-72 and CEA tumor associated antigens in a high percentage of human carcinomas. Our studies have focused on understanding the role of these molecules in tumorigenesis as well as understanding the molecular mechanisms involved in the regulation of expression of these antigens. Specific probes for members of the CEA gene family will be used to study their expression in various tissues and cell lines and to examine the mechanism of regulation of CEA expression by interferons as well as other biological response modifiers. In addition, the gene encoding CEA will be cloned and transfected into mouse metastatic cell lines in order to obtain a mouse model for the study of anti- tumor agents. Polyclonal antisera to deglycosylated TAG-72 and monoclonal antibodies to native TAG-72 will be used to obtain clones from cDNA libraries. Positive clones will be sequenced and used for analysis of TAG-72 expression in normal and tumor cell line and tissues.