MOLECULAR CLONING OF TUMOR ASSOCIATED ANTIGENS

肿瘤相关抗原的分子克隆

基本信息

项目摘要

The use of monoclonal antibody technology has allowed the identification and partial characterization of human carcinoma- associated antigens as well as differentiation antigens of mammary and colon epithelium. MAbs provide powerful tools for use in the diagnosis and management of human carcinomas. They have been used for screening and diagnostic purposes for the detection of tumor associated antigens in blood serum assays, and for the radiolocalization of primary and metastatic carcinoma lesions in-situ. Using radiolabeled monoclonal immunoglobulins and fragments, a powerful therapy regime is being developed. The overall goal of this project is to molecularly clone and identify the genes that encode the tumor associated antigens TAG-72 and CEA. We have recently initiated studies for the cloning of TAG-72. These studies include: 1) the construction of cDNA libraries from LS174 mRNA in several prokaryotic and eukaryotic expression vectors. 2) the cotransfection of LS174 tumor DNA with plasmids carrying drug selectable markers into mammalian cells. 3) the construction of oligomeric probes to the peptide sequence of TAG-72 for use as hybridization probes. Additional studies have recently been initiated to study the regulation of CEA expression in colon and breast tumor cell lines after interferon induction. Several DNA probes have been developed to use in Northern blot analysis of poly A selected mRNA from these cell lines.
单克隆抗体技术的使用使得

项目成果

期刊论文数量(0)
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J KANTOR其他文献

J KANTOR的其他文献

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{{ truncateString('J KANTOR', 18)}}的其他基金

ISOLATION AND CHARACTERIZATION OF GENES CODING FOR CARCINOMA-ASSOCIATED ANTIGENS
癌相关抗原基因编码的分离和表征
  • 批准号:
    3813405
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
  • 批准号:
    2468459
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
ACTIVE IMMUNOTHERAPY TO HUMAN CARCINOMA ASSOCIATED ANTIGENS
针对人类癌相关抗原的主动免疫治疗
  • 批准号:
    3808563
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
ACTIVE IMMUNOTHERAPY TO HUMAN CARCINOMA ASSOCIATED ANTIGENS
针对人类癌相关抗原的主动免疫治疗
  • 批准号:
    3796509
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
  • 批准号:
    6100941
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
  • 批准号:
    3752070
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
  • 批准号:
    6161041
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
  • 批准号:
    5200983
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
  • 批准号:
    3774358
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
MOLECULAR CLONING AND ANALYSIS OF TUMOR-ASSOCIATED ANTIGENS
肿瘤相关抗原的分子克隆和分析
  • 批准号:
    3916369
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

相似海外基金

CARCINOEMBRYONAL ANTIGEN IDIOTYPE VACCINE
癌胚抗原独特型疫苗
  • 批准号:
    3493386
  • 财政年份:
    1993
  • 资助金额:
    --
  • 项目类别:
METABOLISM OF CARCINOEMBRYONAL ANTIGEN
癌胚抗原的代谢
  • 批准号:
    2091525
  • 财政年份:
    1986
  • 资助金额:
    --
  • 项目类别:
METABOLISM OF CARCINOEMBRYONAL ANTIGEN
癌胚抗原的代谢
  • 批准号:
    3187250
  • 财政年份:
    1986
  • 资助金额:
    --
  • 项目类别:
METABOLISM OF CARCINOEMBRYONAL ANTIGEN
癌胚抗原的代谢
  • 批准号:
    2091524
  • 财政年份:
    1986
  • 资助金额:
    --
  • 项目类别:
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