MOLECULAR CLONING OF TUMOR ASSOCIATED ANTIGENS

肿瘤相关抗原的分子克隆

• 批准号: 3939340
• 负责人: J KANTOR
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939340
• 关键词: breast neoplasms; carcinoembryonal antigen; carcinoma; complementary DNA; genetic library; immunogenetics; immunoglobulins; messenger RNA; molecular cloning; monoclonal antibody; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplasm /cancer radionuclide diagnosis; plasmids; serum; tissue /cell culture; tumor antigens

The use of monoclonal antibody technology has allowed the identification and partial characterization of human carcinoma- associated antigens as well as differentiation antigens of mammary and colon epithelium. MAbs provide powerful tools for use in the diagnosis and management of human carcinomas. They have been used for screening and diagnostic purposes for the detection of tumor associated antigens in blood serum assays, and for the radiolocalization of primary and metastatic carcinoma lesions in-situ. Using radiolabeled monoclonal immunoglobulins and fragments, a powerful therapy regime is being developed. The overall goal of this project is to molecularly clone and identify the genes that encode the tumor associated antigens TAG-72 and CEA. We have recently initiated studies for the cloning of TAG-72. These studies include: 1) the construction of cDNA libraries from LS174 mRNA in several prokaryotic and eukaryotic expression vectors. 2) the cotransfection of LS174 tumor DNA with plasmids carrying drug selectable markers into mammalian cells. 3) the construction of oligomeric probes to the peptide sequence of TAG-72 for use as hybridization probes. Additional studies have recently been initiated to study the regulation of CEA expression in colon and breast tumor cell lines after interferon induction. Several DNA probes have been developed to use in Northern blot analysis of poly A selected mRNA from these cell lines.

单克隆抗体技术的使用使得 鉴定和部分表征人癌- 相关抗原以及 乳腺和结肠上皮。 MAb提供了强大的工具， 在人类癌症的诊断和处理中的用途。 他们 用于筛查和诊断目的， 在血清测定中检测肿瘤相关抗原，和 用于原发性和转移性癌的放射定位 原位损伤。 使用放射性标记的单克隆免疫球蛋白 和碎片，正在开发一种强大的治疗方案。 该项目的总体目标是进行分子克隆， 鉴定编码肿瘤相关抗原的基因 TAG-72和CEA。 我们最近开始研究 TAG-72的克隆。 这些研究包括：1） 来自几种原核和非原核细胞中LS 174 mRNA的cDNA文库 真核表达载体。 2)LS 174共转染 肿瘤DNA与携带药物选择性标记的质粒结合， 哺乳动物细胞 3)寡聚探针的构建 TAG-72的肽序列用作杂交探针。 最近又开始了其他研究， 结肠癌和乳腺癌细胞系中CEA表达的调节 干扰素诱导后。 几种DNA探针已经被 开发用于选择的poly A的北方印迹分析 来自这些细胞系的mRNA。