MOLECULAR CLONING OF TUMOR ASSOCIATED ANTIGENS
肿瘤相关抗原的分子克隆
基本信息
- 批准号:3939340
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:breast neoplasms carcinoembryonal antigen carcinoma complementary DNA genetic library immunogenetics immunoglobulins messenger RNA molecular cloning monoclonal antibody neoplasm /cancer immunodiagnosis neoplasm /cancer immunology neoplasm /cancer radionuclide diagnosis plasmids serum tissue /cell culture tumor antigens
项目摘要
The use of monoclonal antibody technology has allowed the
identification and partial characterization of human carcinoma-
associated antigens as well as differentiation antigens of
mammary and colon epithelium. MAbs provide powerful tools for
use in the diagnosis and management of human carcinomas. They
have been used for screening and diagnostic purposes for the
detection of tumor associated antigens in blood serum assays, and
for the radiolocalization of primary and metastatic carcinoma
lesions in-situ. Using radiolabeled monoclonal immunoglobulins
and fragments, a powerful therapy regime is being developed.
The overall goal of this project is to molecularly clone and
identify the genes that encode the tumor associated antigens
TAG-72 and CEA. We have recently initiated studies for the
cloning of TAG-72. These studies include: 1) the construction of
cDNA libraries from LS174 mRNA in several prokaryotic and
eukaryotic expression vectors. 2) the cotransfection of LS174
tumor DNA with plasmids carrying drug selectable markers into
mammalian cells. 3) the construction of oligomeric probes to the
peptide sequence of TAG-72 for use as hybridization probes.
Additional studies have recently been initiated to study the
regulation of CEA expression in colon and breast tumor cell lines
after interferon induction. Several DNA probes have been
developed to use in Northern blot analysis of poly A selected
mRNA from these cell lines.
单克隆抗体技术的使用使得
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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J KANTOR的其他文献
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{{ truncateString('J KANTOR', 18)}}的其他基金
ISOLATION AND CHARACTERIZATION OF GENES CODING FOR CARCINOMA-ASSOCIATED ANTIGENS
癌相关抗原基因编码的分离和表征
- 批准号:
3813405 - 财政年份:
- 资助金额:
-- - 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
- 批准号:
2468459 - 财政年份:
- 资助金额:
-- - 项目类别:
ACTIVE IMMUNOTHERAPY TO HUMAN CARCINOMA ASSOCIATED ANTIGENS
针对人类癌相关抗原的主动免疫治疗
- 批准号:
3808563 - 财政年份:
- 资助金额:
-- - 项目类别:
ACTIVE IMMUNOTHERAPY TO HUMAN CARCINOMA ASSOCIATED ANTIGENS
针对人类癌相关抗原的主动免疫治疗
- 批准号:
3796509 - 财政年份:
- 资助金额:
-- - 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
- 批准号:
6100941 - 财政年份:
- 资助金额:
-- - 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
- 批准号:
3752070 - 财政年份:
- 资助金额:
-- - 项目类别:
DESIGN OF LIVE RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY
用于主动特异性免疫治疗的重组活疫苗的设计
- 批准号:
6161041 - 财政年份:
- 资助金额:
-- - 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
- 批准号:
5200983 - 财政年份:
- 资助金额:
-- - 项目类别:
RECOMBINANT VACCINES FOR ACTIVE SPECIFIC IMMUNOTHERAPY OF HUMAN CARCINOMA
用于人类癌症主动特异性免疫治疗的重组疫苗
- 批准号:
3774358 - 财政年份:
- 资助金额:
-- - 项目类别:
MOLECULAR CLONING AND ANALYSIS OF TUMOR-ASSOCIATED ANTIGENS
肿瘤相关抗原的分子克隆和分析
- 批准号:
3916369 - 财政年份:
- 资助金额:
-- - 项目类别: