MONOCLONAL ANTIBODIES DEFINE CARCINOMA ASSOCIATED AND DIFFERENTIATION ANTIGENS

单克隆抗体定义癌相关抗原和分化抗原

• 批准号: 3916281
• 负责人: J SCHLOM
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916281
• 关键词: antibody formation; breast neoplasms; carcinoembryonal antigen; carcinoma; cell differentiation; cellular oncology; flow cytometry; histochemistry /cytochemistry; hybridomas; laboratory mouse; metastasis; monoclonal antibody; neoplasm /cancer immunology; tumor antigens

These studies involve the generation and utilization of monoclonal antibodies (MAbs) to identify and characterize human carcinoma associated antigens and differentiation antigens of mammary and colonic epithelium. These MAbs are being used to better understand the cell biology and pathogenesis of human carcinomas, and to provide reagents for use in several aspects of the management of several human carcinomas. These include: detection of occult tumor cells, further defining the degree of differentiation of carcinoma cell populations, serum antigen assays, and radiolocalization of primary and metastatic carcinoma lesions in situ (and potentially therapy) using radiolabeled monoclonal immunoglobulins. These studies are divided into four areas of investigation: (I) The generation and characterization of an MAb that defines a novel tumor associated antigen (TAG-72) (II) The development and characterization of MAbs to a repertoire of epitopes on carcinoembryonic antigen (CEA) which are differentially expressed among carcinoma cell populations, (III) The definition and characterization of breast and colon associated antigens and (IV) The generation and characterization of B72.3 second generation antibodies to further define the TAG-72 antigen and for potential utility in carcinoma management.

这些研究涉及到克隆的产生和利用。 用于识别和表征人类肿瘤的抗体(单抗) 乳腺和乳腺的相关抗原和分化抗原 结肠上皮。这些单抗被用来更好地了解 人类癌症的细胞生物学和发病机制，以及 提供用于管理的几个方面的试剂 几种人类癌症。这些包括：隐匿性肿瘤的检测 细胞，进一步定义肿瘤的分化程度 细胞数量、血清抗原分析和放射定位 原发和转移性癌原位病变(和潜在的 治疗)使用放射性标记的单抗免疫球蛋白。这些 研究分为四个调查范畴：(I) 定义小说的单抗的产生和表征 肿瘤相关抗原(TAG-72)(II)的发展和 单抗对表位的鉴定 差异表达的癌胚抗原(CEA) 在癌细胞群体中，(Iii)定义和 乳房和结肠相关抗原的特性和(IV) 第二代B72.3菌株的产生及特性 进一步确定TAG-72抗原和潜在功能的抗体 在癌症治疗中的应用。