BIOLOGICAL AND BIOCHEMICAL STUDIES OF ANTIGENS ON MALARIA-INFECTED RED CELLS

疟疾感染红细胞抗原的生物学和生化研究

• 批准号: 3822004
• 负责人: R J HOWARD
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822004
• 关键词: Africa; Plasmodium falciparum; Plasmodium knowlesi; antibody formation; cell adhesion; cellular immunity; cytoskeleton; erythrocyte membrane; erythrocytes; histidine; host organism interaction; immunoelectron microscopy; intracellular parasitism; lactoperoxidase; malaria; membrane activity; microorganism immunology; protozoal antigen; protozoal infection; radiotracer; surface antigens; tissue /cell culture; vascular endothelium

We are studying the structure and function of malarial proteins inserted into and through the membrane of erythrocytes infected with mature asexual malaria parasites and molecules on the endothelial cells involved in cytoadherence. Five malarial proteins have been localized to the membrane of Plasmodium falciparum-infected erythrocytes at the mature trophozoite stage. The Mr approximately 300,000 cell surface protein associated with the property of cytoadherence to endothelial cells (denoted Pf EMP 1, P. falciparum erythrocyte membrane protein 1) has been shown to be antigenically distinct from another very large (Mr approximately 300,000) malarial protein located under the surface membrane in the electron-dense material at knobs (denoted Pf EMP 2). The histidine-rich protein (HRP) associated with knob expression (denoted PfHRP 1) was also shown to be localized under the membrane in the electron- dense material. Another HRP that is exported from infected cells into plasma (called Pf HRP 2) is also associated with the surface membrane, presumably en route for secretion since it is accessible to antibody at the cell surface and also attached to the cytoskeleton. We also showed that RESA, a protein inserted into the host membrane during merozoite invasion, remains in the membrane and is accessible to lactoperoxidase catalyzed radioiodination with trophozoite-infected cells.

我们正在研究疟疾蛋白质的结构和功能 插入并穿过感染的红细胞膜 与成熟的无性疟疾寄生虫和分子 内皮细胞参与细胞粘附。 五种疟疾蛋白质已经定位于 恶性疟原虫感染的成熟红细胞 滋养体阶段 大约30万个细胞表面 与细胞粘附特性相关的蛋白质， 内皮细胞（表示为Pf EMP 1、恶性疟原虫红细胞 膜蛋白1）已被证明是抗原性不同的 从另一个非常大的（约30万）疟疾 蛋白质位于表面膜下的电子致密 旋钮处的材料（表示为Pf EMP 2）。 富含组氨酸的蛋白 (HRP)与结表达相关的蛋白质（表示为PfHRP 1）是 也被证明是位于膜下的电子- 致密材料 另一种从受感染细胞输出的HRP 进入血浆（称为Pf HRP 2）也与表面 膜，大概是在途中分泌，因为它是 在细胞表面可接近抗体，并且还附着到 细胞骨架 我们还表明，RESA，一种插入到 裂殖子侵入时宿主细胞膜， 膜，并可接近乳过氧化物酶催化 用滋养体感染的细胞进行放射性碘标记。