RABBIT ALLOTYPES--STRUCTURE, ORGANIZATION AND REGULATED EXPRESSION OF IG GENES

我们利用基因工程技术研究兔免疫系统的基因 分子生物学。我们使用了一个限制片段长度 兔T细胞受体β链的多态(RFLP) 兔的恒定区基因(Cβ)和一个RFLP 免疫球蛋白(Ig)轻链(C Kappa)测定 兔的Cβ和C kappa没有遗传联系，因为它们 在老鼠体内，但像人类一样独立分离。C-kappa 另一方面，RFLP与基因的表达密切相关 K2basl-Ig同种异型及新发现的兔V-RFLP 卡帕基因。到目前为止，还没有确定重组体。我们 发现了序列和连锁的进化保守性 编码T细胞受体β的基因片段之间的关系 链状可变区。三只紧密相连的兔子中的两只 小鼠和小鼠的基因具有紧密相连的同源基因 天哪。第三个兔Vβ基因与人类强烈杂交 DNA兔子探头应该识别并允许测绘 此前未知的人类Vβ基因。像其他物种一样， 一些兔子有两个相似的Cβ基因，但我们发现 其他人有三种异型的Cβ1和Cβ 2.Cβ1的5‘-嘌呤和嘧啶交替延伸 外显子1，外显子1中的完美CHI序列，且CHI带有一个 Cβ外显子1的内含子3‘不匹配，围绕 异型位置，可能促成了 Cβ1和Cβ1中相似的同型氨基酸序列 通过基因转化获得个体兔(同种异型)的β2- 就像机械装置。

We study genes of the rabbit immune system by techniques of molecular biology. We used a restriction fragment length polymorphism (RFLP) of the rabbit T cell receptor beta chain constant region genes (C beta) and an RFLP of rabbit immunoglobulin (Ig) kappa light chains (C kappa) to determine that rabbit C beta and C kappa are not genetically linked as they are in mice, but segregate independently as in man. The C kappa RFLP on the other hand is closely linked to the expression of the K2basl Ig allotype and to a newly discovered RFLP of rabbit V kappa genes. No recombinants have been identified to date. We found evolutionary conservation of both sequence and linkage relationships of gene segments encoding T cell receptor beta chain variable regions. Two of three closely linked rabbit V beta genes have homologs that are closely linked in both mouse and man. A third rabbit V beta gene hybridizes strongly to human DNA. The rabbit probe should identify and allow mapping of a previously unidentified human V beta gene. Like other species, some rabbits have two similar C beta genes but we found that others have three and allotypic forms of both C beta 1 and C beta 2. Stretches of alternating purines and pyrimidines 5' of C beta 1 exon 1, a perfect Chi sequence in exon 1, and Chi with one mismatch in the intron 3' of C beta exon 1, surrounding the allotypic positions, may have contributed to the generation of similar allotypic amino acid sequences in both C beta 1 and C beta 2 of individual rabbits (isoallotypes) by a gene-conversion- like mechanism.