IG GENETICS--ONTOGENY AND DIFFERENTIATION OF CELLS OF THE RABBIT IMMUNE SYSTEM

我们利用免疫遗传学和分子生物学技术来研究 兔免疫球蛋白(Ig)和相关基因的重组等 激活基因所必需的RAG-1和RAG-2 在淋巴细胞发育过程中发生重排。我们调查了 阑尾、毛囊和生发等解剖部位的发育 肠道相关淋巴组织中的中心及其调控表达 淋巴细胞发育过程中免疫球蛋白基因序列的多样性。 兔B细胞上的膜IGD尚未被明确鉴定； 本应包含Delta基因的遗传区已被克隆，但 与Delta重链外显子对应的序列尚未被 已确认身份。我们已经鉴定出两种表面免疫球蛋白复合体 兔外周血中具有类似重链的B细胞 表观分子大小证实了先前关于存在一种 兔B细胞上表达的第二表面受体可能是IgD或AN 免疫球蛋白相关分子。与一种或两种非共价结合 这些受体是几种蛋白质，是表面的成分 兔B细胞表达免疫球蛋白(MiG)复合体。三 发现与IgM相关的蛋白质(42 kDa、37 kDa、36 kDa) 表达于外周血和脾的B细胞上。这些蛋白质可能 是小鼠Ig-β(B29)、Ig-Gamma(截短B29)的兔同系物 和Ig-α(mb-1)。凝集素让我们可以解剖兔的阑尾 基于局部将卵泡和生发中心划分为亚室 糖基化细胞表面受体的表达。我们发现了另外两个 含36 kDa和34 kDa蛋白的免疫球蛋白相关蛋白复合体 从阑尾分离的单个毛囊的裂解物。这个 阑尾可能在发育早期起作用，就像鸡法氏囊或 绵羊回肠佩耶氏区。然而，兔的阑尾并不是渐开的。 但可能改变其功能，成为人体内的次级淋巴器官。 成年人。

We use techniques of immunogenetics and of molecular biology to study the rabbit immunoglobulins (Igs), and related genes such as the recombination activating genes RAG-1 and RAG-2 which are necessary for gene rearrangements to occur during lymphocyte development. We investigate the development of anatomical sites such as appendix follicles and germinal centers in gut associated lymphoid tissues and the regulated expression and sequence diversification of Ig genes during lymphoid cell development. Membrane IgD has not been definitively identified on rabbit B cells; the genetic region which should contain the delta gene has been cloned, but sequences corresponding to delta heavy chain exons have not been identified. We have identified two surface immunoglobulin complexes on rabbit peripheral blood B cells that possess heavy chains of similar apparent molecular size confirming previous reports of the presence of a second surface receptor expressed on rabbit B cells which may be IgD or an IgM-related molecule. In noncovalent association with one or both of these receptors are several proteins that are components of surface immunoglobulin (mIg) complexes expressed on rabbit B cells. Three proteins (42 kDa, 37kDa, 36kDa) were found in association with IgM expressed on B cells from peripheral blood and spleen. These proteins may be the rabbit homologues of murine Ig-beta (B29), Ig-gamma (truncated B29) and Ig-alpha (mb-1). Lectins are allowing us to dissect rabbit appendix follicles and germinal centers into subcompartments based on local expression of glycosylated cell surface receptors. We found two additional IgM-associated protein complexes containing 36kDa and 34kDa proteins in lysates from individual follicles isolated from the appendix. The appendix may be functioning early in development like the chicken bursa or sheep ileal Peyer's patch. However, the rabbit appendix does not involute but may alter its function and become a secondary lymphoid organ in the adult.