TRANSFORMATION BY HUMAN CMV AND HHV-6

人巨细胞病毒(HCMV)与多种肿瘤有关 疾病，包括前列腺癌，结肠腺癌， 宫颈癌和卡波西肉瘤。形态变换 CMV Towne的区域II(MTR II)之前已本地化为980 含有三个推测的开放阅读框(ORF)的碱基对片段 由79、83和34个氨基酸组成。菌株中存在同源区域 汤恩，公元169年和田中。然而，田中mtrii减少了转型。 与汤恩和AD169相比，活动。有趣的是，79个氨基酸 Towne和AD169中都存在的酸性ORF在田中被截断， 这表明，这可能是高效转型的关键。 两个功能启动子(P1和P2)已经在Towne中被定位 人巨细胞病毒mtrII.79个氨基酸的ORF可以从P1翻译而来 转录本，而83和34个氨基酸的ORFs可以从 都不是成绩单。检测到类似的功能启动子活性 来自HCMV的MTRII共线变换和非变换区域内 菌株AD169和田中。两个启动子都不是菌株 汤恩表现出转化活性。结合之前的数据， 这些数据表明，人巨细胞病毒mtrII的转化不是通过 启动子插入，但需要完整的79个氨基酸的ORF。

Human cytomegalovirus (HCMV) has been associated with several neoplastic diseases, including prostatic carcinoma, adenocarcinoma of the colon, cervical carcinoma, and Kaposi's sarcoma. The morphological transforming region II (mtrII) of CMV Towne has previously been localized to a 980 base-pair fragment containing three putative open reading frames (ORFs) of 79, 83, and 34 amino acids (aa). Homologous regions exist in strains Towne, AD169 and Tanaka. However, Tanaka mtrII has reduced transforming activity when compared to Towne and AD169. Interestingly, the 79 amino acid ORF present in both Towne and AD169 is truncated in Tanaka, indicating that it may be crucial for efficient transformation. Two functional promoters (P1 and P2) have been mapped within the Towne HCMV mtrII. The 79 amino acid ORF can be translated from the P1 transcript, while the 83 and 34 amino acid ORFs could be translated from either transcript. Similar functional promoter activities were detected within mtrII colinear transforming and non-transforming regions from HCMV strains AD169 and Tanaka, respectively. Neither promoter from strain Towne exhibited transforming activity. Combined with previous data, these data suggest that transformation by HCMV mtrII does not occur by promoter insertion but requires an intact 79 amino acid ORF.