TRANSCRIPTION ANALYSIS OF THE JC VIRUS ENHANCER

JC 病毒增强子的转录分析

• 批准号: 3874659
• 负责人: J BRADY
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874659
• 关键词: DNA binding protein; HeLa cells; Polyomavirus hominis 2; cell differentiation; crosslink; embryo /fetus tissue /cell culture; gel electrophoresis; gel filtration chromatography; gene expression; genetic enhancer element; genetic manipulation; genetic mapping; genetic transcription; genetically modified animals; glia; glioma; human immunodeficiency virus; human tissue; laboratory mouse; molecular weight; neoplasm /cancer genetics; neuroblastoma; nucleic acid sequence; oligonucleotides; oncoproteins; progressive multifocal leukoencephalopathy; retinoate; transcription factor; transfection; viral carcinogenesis; virus DNA; virus genetics; virus protein; virus replication; virus virus interaction

JC virus (JCV) is an oncogenic human papovavirus that has been postulated to be involved in the formation of glial tumors in patients with progressive multifocal leukoencephalopathy (PML). The incidence of PML is increased significantly in AIDS patients. It was of interest, therefore, to investigate the interaction of HIV and JCV. We initiated these studies by analyzing the effect of the HIV tat gene product on JCV viral gene expression. Using a combination of cotransfection assays and in vitro DNA binding assays, we have demonstrated that tat activates early gene expression at the transcriptional level. At least part of the increase in transcription is a result of activation of transcription factors which interact with the tissue-specific JCV enhancer. Investigations are underway to determine if the activation is due to the increased synthesis or change in specific activity of existing transcription factors.

JC病毒（JCV）是一种被认为是致癌的人乳头状病毒