SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
批准号:
3839193
负责人:
B J BAUM
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
G protein acinar cell alpha adrenergic receptor anticholinergic agent biological signal transduction calcium cyclic AMP inositol phosphates laboratory rat muscarinic receptor neurochemistry neurotransmitters parotid gland phospholipase C quinuclidines receptor coupling saliva second messengers secretion tissue /cell culture
中文摘要
口腔的健康是由唾液分泌物维持的。公关
英文摘要
The health of the oral cavity is maintained by salivary secretions. The pr
cipal
function of salivary glands is to produce these complex fluids. We utilize
n vitro
dispersed cells of salivary glands to understand mechanisms controlling sal
a formation.
We have focused our studies on neurotransmitter regulation of secretory eve
s and
associated signalling mechanisms. During this reporting period the primary
ocus of study
continues to be muscarinic receptors (mAChRs) in rat parotid gland acinar c
ls and their
coupling to functional responses via specific G proteins. We have also ini
ated studies
of alpha-adrenergic receptors (alpha1-ARs) in these cells. In parotid cell
stimulation
of mAChRs results in the generation of inositol phosphates via the activati
of a
phosphatidylinositol 4,5-bisphosphate specific phospholipase C. Subsequent
this
response leads to the elevation of cytosolic Ca2+ levels and fluid secretio
Additionally, mAChRs can mediate the inhibition of agonist induced cAMP for
tion. We
have characterized the binding of a subtype non-selective antagonist (quinu
idinyl
benzilate, QNB) to mAChRs in intact rat parotid cells. Specific binding is
aturable,
time- and temperature-dependent (Bmax approximately 80 fmol/mg protein; Kd
proximately
100 pM). Also, we have assessed the relationship between mAChR occupancy a
second
messenger formation, determining that a moderate population (approximately
-40%) of
spare receptors exist for inositol trisphosphate (IP3) formation. We previ
sly reported
that rat parotid M3-mAChRs couple to two different signal transducing G pro
ins. This
year we determined the nucleotide sequence encoding the region of this rece
or gene
involved in G-protein coupling and we observed that it is virtually identic
to other
reported 3 sequences. Thus, the mAChR itself is unlikely to be responsibl
for the
divergent coupling. Additionally, we have shown that the alpha1-adrenergic
eceptor
subtype involved in the fluid secretory response is alpha1A.
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:2572315
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:6161783
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:5201768
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3875207
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3775656
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3939949
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3963723
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:4692638
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3753528
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3854193
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:B J BAUM
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依托单位:
海外基金