SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
批准号:
3839193
负责人:
B J BAUM
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
G protein acinar cell alpha adrenergic receptor anticholinergic agent biological signal transduction calcium cyclic AMP inositol phosphates laboratory rat muscarinic receptor neurochemistry neurotransmitters parotid gland phospholipase C quinuclidines receptor coupling saliva second messengers secretion tissue /cell culture
中文摘要
口腔的健康是靠唾液分泌维持的。媒体
辅助体
唾液腺的功能就是产生这些复杂的液体。我们利用
N体外
分散的唾液腺细胞以了解控制唾液流失的机制
一个队形。
我们的研究重点是分泌性前夕的神经递质调节。
S和
相关的信令机制。在本报告所述期间,主要
研究的重点
大鼠腮腺腺泡内M受体的表达
LS和他们的
通过特定的G蛋白与功能反应相偶联。我们也有INI
专门化研究
这些细胞中的α-肾上腺素能受体(α1-ARs)。在腮腺细胞中
刺激
MAChRs的活性导致肌醇磷酸盐的生成
一种
磷脂酰肌醇4,5-二磷酸特异性磷脂酶C
这
反应导致细胞内钙离子水平和液体分泌增加
此外,mAChRs还可介导激动剂诱导的cAMP抑制
提顿。我们
已经表征了一种亚型非选择性拮抗剂(QUU)的结合
乙二酰亚胺
对完整的大鼠腮腺细胞的mAChRs。特定的绑定是
可满足的,
依赖时间和温度(Bmax约为80fmol/mg蛋白质;Kd
最接近
晚上10点)。此外,我们还评估了mAChR占有率与
第二
信使编队,确定适度的人口(大约
-40%)
三磷酸肌醇(IP3)的形成存在备用受体。我们占上风
狡猾的报道
大鼠腮腺M3-mAChRs与两种不同信号转导G蛋白偶联
移民局。这
年,我们测定了编码该基因区域的核苷酸序列
或基因
参与G蛋白偶联,我们观察到它实际上是相同的
给其他人
报道了3个序列。因此,mAChR本身不太可能负责
对于
发散耦合。此外,我们已经证明了α1-肾上腺素能
感受器
参与液体分泌反应的亚型是α1a。
英文摘要
The health of the oral cavity is maintained by salivary secretions. The pr
cipal
function of salivary glands is to produce these complex fluids. We utilize
n vitro
dispersed cells of salivary glands to understand mechanisms controlling sal
a formation.
We have focused our studies on neurotransmitter regulation of secretory eve
s and
associated signalling mechanisms. During this reporting period the primary
ocus of study
continues to be muscarinic receptors (mAChRs) in rat parotid gland acinar c
ls and their
coupling to functional responses via specific G proteins. We have also ini
ated studies
of alpha-adrenergic receptors (alpha1-ARs) in these cells. In parotid cell
stimulation
of mAChRs results in the generation of inositol phosphates via the activati
of a
phosphatidylinositol 4,5-bisphosphate specific phospholipase C. Subsequent
this
response leads to the elevation of cytosolic Ca2+ levels and fluid secretio
Additionally, mAChRs can mediate the inhibition of agonist induced cAMP for
tion. We
have characterized the binding of a subtype non-selective antagonist (quinu
idinyl
benzilate, QNB) to mAChRs in intact rat parotid cells. Specific binding is
aturable,
time- and temperature-dependent (Bmax approximately 80 fmol/mg protein; Kd
proximately
100 pM). Also, we have assessed the relationship between mAChR occupancy a
second
messenger formation, determining that a moderate population (approximately
-40%) of
spare receptors exist for inositol trisphosphate (IP3) formation. We previ
sly reported
that rat parotid M3-mAChRs couple to two different signal transducing G pro
ins. This
year we determined the nucleotide sequence encoding the region of this rece
or gene
involved in G-protein coupling and we observed that it is virtually identic
to other
reported 3 sequences. Thus, the mAChR itself is unlikely to be responsibl
for the
divergent coupling. Additionally, we have shown that the alpha1-adrenergic
eceptor
subtype involved in the fluid secretory response is alpha1A.
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:6161783
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财政年份:--
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负责人:B J BAUM
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依托单位:
SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:2572315
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:5201768
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3775656
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3875207
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3939949
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3963723
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3753528
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:4692638
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负责人:B J BAUM
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SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES
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批准号:3854193
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负责人:B J BAUM
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