SALIVARY GLAND SECRETORY MECHANISMS DURING NORMAL AND ALTERED FUNCTIONAL STATES

正常和功能改变状态下的唾液腺分泌机制

• 批准号: 6161783
• 负责人: B J BAUM
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161783
• 关键词: Adenoviridae; acinar cell; epithelium; gene therapy; laboratory rat; recombinant virus; saliva; salivary disorder; salivary glands; salivation; tissue /cell culture; transfection; water channel

The health of the oral cavity is maintained by salivary secretions. Our past studies have focused on understanding mechanisms of saliva formation and their alteration during pathologic processes. During this reporting period we have continued our efforts to utilize gene transfer technology to alter the phenotypic behavior of salivary epithelial cells. Specifically, we have continued efforts to correct the salivary damage suffered by patients whose salivary glands are exposed to radiation during treatment for head and neck cancer. We have extended past in vitro findings and shown that delivery of a recombinant adenovirus encoding aquaporin-1 to irradiated salivary glands in rats can lead to increased fluid secretion to near normal levels. Secondly, we have also extended studies on the systemic secretion of transgene products from transduced salivary epithelial cells. We showed that glands infected with a recombinant adenovirus encoding human growth hormone secrete therapeutic levels of functional hormone in adult rats. We also have demonstrated that administration of recombinant adenoviruses to rat salivary glands does not result in significant systemic toxicity.

口腔的健康是由唾液分泌物维持的。 我们的 过去的研究集中在理解唾液形成的机制上 以及它们在病理过程中的改变。 在此报告中 时期我们继续努力利用基因转移技术 改变唾液上皮细胞的表型行为。 具体来说，我们继续努力纠正唾液损害 受唾液腺暴露于辐射的患者遭受的痛苦 治疗头颈癌。 我们已经在体外扩展了 发现并表明了重组腺病毒编码的递送 水通道蛋白1至大鼠的辐射唾液腺可能导致增加 流体分泌到接近正常水平。 其次，我们也扩展了 转导的转基因产物的系统分泌的研究 唾液上皮细胞。 我们证明了感染了 编码人类生长激素分泌治疗的重组腺病毒 成年大鼠功能激素的水平。 我们也证明了 对大鼠唾液腺的重组腺病毒的给药 不会导致严重的全身毒性。