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NEUROENDOCRINOLOGY OF REPRODUCTION AND GROWTH

生殖和生长的神经内分泌学

基本信息

批准号：
3096641
负责人：
NICHOLAS C LING
金额：
$ 125.72万
依托单位：
WHITTIER INSTITUTE FOR DIABETES & ENDOC
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-03-01 至 1994-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3096641
关键词：
growth factor hormone regulation /control mechanism hypothalamic pituitary axis neuroendocrine system peptide hormone reproduction

项目摘要

Historically, research supported by this Program Project has contributed significantly to our basic understanding of the neuroendocrinological control of reproduction and growth. Continuing with this objective, the current renewal application contains projects aimed at elucidating the complex mechanisms regulating ovarian follicle growth and differentiation. A multidisciplinary approach will be utilized in which regulation of follicular growth and differentiation will be examined at the molecular, cellular and organismic levels. Specifically, projects are proposed to: (1) isolate and identify all the polypeptide growth factors present in follicular fluid as these factors may have inherent autocrine/paracrine roles in regulating folliculogenesis; (2) explore the regulation and intragonadal action of insulin-like growth factor-binding proteins; (3) ascertain the physiological importance of follistatin in reproduction; and (4) study the regulation of follistatin at the gene level. Experiments proposed in this Program Project will be supported by four Core Units. These Core Units will be established to: (A) maintain and improve the methodology for amino acid analysis and microsequencing to characterize polypeptides, as well as maintain the peptide synthesis facility to provide synthetic peptides for all of the research projects for biological studies and raising of antibodies; (B) maintain the molecular biology facility to perform routine DNA sequencing, oligonucleotide synthesis and preparation of ribo-probes for in situ hybridization studies; (C) provide a central facility for the development and performance of radioimmunoassays and (D) provide a centralized administrative core facility to oversee the daily operation of the Program Project.

从历史上看，由该计划项目支持的研究有助于对我们对神经内分泌学的基本理解有重要意义对繁殖和生长的控制。继续实现这一目标，目前的续期申请包含旨在澄清调控卵泡生长和分化的复杂机制。将使用多学科方法，在其中监管卵泡的生长和分化将在分子水平上进行检查，细胞和组织水平。具体而言，拟开展的项目包括： (1)分离和鉴定所有存在于卵泡液，因为这些因素可能有固有的自分泌/旁分泌在调节卵泡发生中的作用；(2)探讨胰岛素样生长因子结合蛋白在性腺内的作用确定卵泡抑素在生殖中的生理重要性；以及 (4)从基因水平研究卵泡抑素的调控作用。实验本计划中提出的项目将由四个核心单位提供支持。设立这些核心单位的目的是：(A)维持和改善氨基酸分析和微序列分析的方法学多肽，以及维持多肽合成设施，以提供用于所有生物学研究项目的合成肽和抗体的提高；(B)维持分子生物学设施以执行常规DNA测序、寡核苷酸合成和制备用于原位杂交研究的核糖体探针；(C)提供中心用于开发和执行放射免疫分析的设施和(D) 提供一个集中的行政核心设施来监督日常计划项目的运行情况。

项目成果

期刊论文数量（196）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Localization of insulin-like growth factor-binding protein-5 messenger ribonucleic acid in rat ovaries during the estrous cycle.

DOI：
10.1210/endo.130.4.1372237
发表时间：
1992-04
期刊：
Endocrinology
影响因子：
4.8
作者：
Gregory F. Erickson;Akira Nakatani;Nicholas Ling;Shunichi Shimasaki
通讯作者：
Gregory F. Erickson;Akira Nakatani;Nicholas Ling;Shunichi Shimasaki

Distribution of growth hormone-releasing hormone-like immunoreactivity in human tissue extracts.

人体组织提取物中生长激素释放激素样免疫反应性的分布。

DOI：
10.1210/jcem-59-2-263
发表时间：
1984
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
0
作者：
Shibasaki,T;Kiyosawa,Y;Masuda,A;Nakahara,M;Imaki,T;Wakabayashi,I;Demura,H;Shizume,K;Ling,N
通讯作者：
Ling,N

Growth hormone-releasing factor from ovine and caprine hypothalamus: isolation, sequence analysis and total synthesis.

来自绵羊和山羊下丘脑的生长激素释放因子：分离、序列分析和全合成。