DEVELOP LIVE ATTENTUATED COLD ADAPTED INFLUENZA VACCINE

合同的目标包括建立程序 用于制备和表征活的、稀释的、冷的- 甲型和乙型流感病毒改良剂，用作疫苗。 在项目官员的指导下，这个实验室将是一个 来自主要现场分离物的临床样本储存库 评估它们作为血凝素和神经氨酸酶的潜在用途 疫苗株的捐赠者。承包商将负责 数据的管理、检索、分析、验证和发布 以及野生和野生动物的编目、储存和运输 研究所需的甲型和乙型流感病毒重新组合。vbl.使用 一个雪貂模型，重新组合的克隆将被描述为 表型和基因的稳定性、致病力和反应性 包括确定适当的剂量和时间表 三价CA重组疫苗鼻腔给药。在……里面 此外，承包商将提供疫苗克隆以分发给 项目干事要求的其他调查人员，并将 在临床试验中对从受试者身上分离的病毒进行鉴定。分子 将进行分析，以进一步确定案件的特征 为病毒提供亲本，并将它们与它们的野生型祖先进行比较。 将继续调查某些人通过什么机制 重组疫苗毒株变得具有反应性，试图更好地 预测疫苗毒株在人类身上的行为。

The objectives of the contract involve the establishment of procedures for the preparation and characterization of live, attenuated, cold- adapted (ca) influenza A and B virus reassortants for use as vaccines. Under the direction of the Project Officer, this laboratory will be a repository for clinical samples from primary field isolates and will evaluate them for potential use as hemagglutinin and neuraminadase donors for the vaccine strains. The Contractor will be responsible for the management, retrieval, analysis, validation and publication of data and the cataloguing, storage, and shipment of the wild-type and reassortant influenza A and B viruses required for the studies. Using a ferret model, the reassortant clones will be characterized as to phenotypic and genotypic stability, virulence, and reactogenicity including the determination of the appropriate dose and schedule for intranasal administration of trivalent ca reassortant vaccines. In addition, the Contractor will supply vaccine clones for distribution to other investigators as requested by the Project Officer and will characterize virus isolated from subjects in clinical tests. Molecular analyses will be conducted in order to further characterize the ca parent viruses and compare them to their wild-type progenitors. Investigations will continue into the mechanisms by which certain of the reassortant vaccine strains become reactogenic in an attempt to better predict the behavior of the vaccine strains in humans.