DEVELOP LIVE ATTENUATED COLD-ADAPTED INFLUENZA VACCINE

开发冷适应流感减毒活疫苗

• 批准号: 3594538
• 负责人: H F MAASSAB
• 金额: $ 39.55万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-10-25 至 1989-10-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3594538
• 关键词: attenuated microorganism; ferrets; immunological substance; influenza vaccines; virus cytopathogenic effect; virus genetics; virus morphology

Provide cold-adapted reassortant vaccines of influenza virus types A and B with the relevant surface antigens for use inman. Identify a stable, high yielding donor strain for use in producing type B reassortant vaccines. Derive and characterize reassortants of both type A and B ca donor viruses with single genes from these ca patients. Test isolatesfrom field vaccine trials for anomalies in biological markers and subsequently in genotypes. Provide and sequence recombinant DNA clones of both types A and B donor genes. Furnish baseline data from the use of bivalent and polyvalentca vaccine preparations in an animal model for application in man. The approaches are designed to establish the geneticmechanisms of cold adaptation and attenuation. The efforts necessary to achieve these objectives will be performed withcost effectiveness along guidelines for the eventual licensing of these vaccines.

提供甲型和B型流感病毒冷适应性疫苗 与人体内使用的相关表面抗原一起。 确定一个稳定、高 产生用于生产B型疫苗的供体菌株。 获得并表征A型和B型ca供体病毒的复制物 这些CA患者的单个基因。 来自田间疫苗的试验分离株 在生物标记和随后的基因型异常的试验。 提供A型和B型供体的重组DNA克隆并测序 基因. 提供使用二价和多价药物的基线数据c 在动物模型中应用于人的疫苗制剂。 这些方法旨在建立寒冷的遗传机制这些方法旨在建立寒冷的遗传机制 适应和衰减。 实现这些目标所需的努力 目标将按照成本效益沿着指导方针执行， 这些疫苗的最终许可。

项目成果

A 36 nucleotide deletion mutation in the coding region of the NS1 gene of an influenza A virus RNA segment 8 specifies a temperature-dependent host range phenotype.

• DOI: 10.1016/0168-1702(90)90014-3
• 发表时间: 1990
• 期刊: Virus research
• 影响因子: 5
• 作者: M. Snyder;W. London;H. F. Maassab;R. Chanock;B. Murphy

Cold-adapted influenza viruses for use as live vaccines for man.

冷适应流感病毒用作人类活疫苗。

• 发表时间: 1990
• 期刊: Advances in biotechnological processes
• 作者: Maassab,HF;Heilman,CA;Herlocher,ML
• 通讯作者: Herlocher,ML

Genetics of cold-adapted B/Ann Arbor/1/66 influenza virus reassortants: the acidic polymerase (PA) protein gene confers temperature sensitivity and attenuated virulence.

冷适应 B/Ann Arbor/1/66 流感病毒重配体的遗传学：酸性聚合酶 (PA) 蛋白基因赋予温度敏感性和减弱的毒力。

• DOI: 10.1016/0882-4010(87)90068-4
• 发表时间: 1987
• 期刊: Microbial pathogenesis
• 影响因子: 3.8
• 作者: Donabedian,AM;DeBorde,DC;Maassab,HF
• 通讯作者: Maassab,HF

Differential interaction of virulent and attenuated influenza virus strains with ferret alveolar macrophages: possible role in pathogenicity.

强毒和减毒流感病毒株与雪貂肺泡巨噬细胞的不同相互作用：在致病性中的可能作用。

• DOI: 10.1093/infdis/161.4.699
• 发表时间: 1990
• 期刊: The Journal of infectious diseases
• 作者: Riser,BL;Maassab,HF
• 通讯作者: Maassab,HF

Nucleotide sequences of the PA and PB1 genes of B/Ann Arbor/1/66 virus: comparison with genes of B/Lee/40 and type A influenza viruses.

B/Ann Arbor/1/66 病毒的 PA 和 PB1 基因的核苷酸序列：与 B/Lee/40 和 A 型流感病毒的基因比较。

• DOI: 10.1016/0168-1702(87)90038-4
• 发表时间: 1987
• 期刊: Virus research
• 影响因子: 5
• 作者: DeBorde,DC;Naeve,CW;Herlocher,ML;Maassab,HF
• 通讯作者: Maassab,HF