CD SPECTROMETER

CD光谱仪

• 批准号: 3521306
• 负责人: JEFFREY W NELSON
• 金额: $ 12.2万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-02 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521306
• 关键词: biomedical equipment purchase; circular dichroism; conformation; drug interactions; enzyme mechanism; enzyme structure; intermolecular interaction; protein folding

Funds are requested to purchase a Circular Dichroism (CD) Spectrometer with excellent far UV capabilities. This equipment is needed to fill a significant gap in basic instrumentation. The far UV capabilities are essential for structural studies of biological macromolecules, and these needs cannot be fulfilled by any currently available CD spectrometer. The CD will be used to monitor structural transitions in peptides, structures of trapped protein folding intermediates, structural aspects of protein allosteric transitions, and effects of drugs on DNA structure. The main users include five faculty in the biochemistry department and two faculty in the chemistry department. This instrument will be administered by an internal advisory committee as a shared instrument in the College of Basic Sciences at Louisiana State University.

请拨经费购买圆二色谱仪

项目成果

Site-directed mutagenesis of rabbit muscle phosphofructokinase cDNA. Mutations at glutamine 200 affect the allosteric properties of the enzyme.

兔肌肉磷酸果糖激酶 cDNA 的定点诱变。

• 发表时间: 1993
• 期刊: The Journal of biological chemistry
• 作者: Li,J;Zhu,X;Byrnes,M;Nelson,JW;Chang,SH
• 通讯作者: Chang,SH