权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

HYBRID (EBQQ) MASS SPECTROMETER

混合型 (EBQQ) 质谱仪

基本信息

批准号：
3520993
负责人：
TODD D WILLIAMS
金额：
$ 38.5万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-06-20 至 1992-06-19
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3520993
关键词：
biomedical equipment purchase biomedical equipment resource mass spectrometry

项目摘要

This request is for a Hybrid Mass Spectrometer to be part of the Mass Spectrometry Laboratory at the University of Kansas, serving the Departments of Chemistry, Medicinal Chemistry, Pharmaceutical Chemistry, Pharmacology and Toxicology, KU Medical Center and the centers for Biomedical and Bioanalytical Research. The primary need is for increased fast atom bombardment (FAB) capability. This has been an established service lab with growing capability and sample load for 10 years. A total of thirty groups currently use the lab and at 7/12 through the fiscal year the lab has run 1690 samples and will easily reach 2500 by July. This work is being carried out on three facility instruments,a Varian-NUT CH5, a Nermag quadrupole GC/MS system, and a high resolution VG ZAB HS. The Nermag and the ZAB are used for the bulk of the samples. The CH5 (bought in 1970) is obsolete as a FAB instrument. The Nermag is currently saturated in use by probe and GC experiments. The ZAB has reached near saturation with routine exact mass and FAB analysis. Starting in July of 1989 the demand for FAB analysis has increased 6 times from the previous year. FAB work is expected to double again as peptide molecular weight experiments are done in support of four groups just beginning to use mass spectrometry for peptide characterization. The peptide projects involve the identification and localization of modified amino acids in small peptides. The MS/MS capability of the hybrid will be used to provide the sequence information not available in the FAB experiments. The unit mass precursor and fragment ion resolution obtainable with a hybrid in CAD experiments will be vital for peptide projects involving bromine and deuterium labeled peptides. Two groups are currently synthesizing coordination compounds and have been the major FAB users for confirmation of intermediates and final products. Both groups have had mixed success in obtaining molecular ions from their metal containing samples. Frequently demetallated species are observed in the FAB spectra. Similar compounds have been characterized by Field Desroption (FD). The use of FD for coordination compounds provides a soft ionization method that avoids the complications of matrix chemistry.

本申请要求将混合质谱仪作为质谱仪的一部分，堪萨斯大学光谱实验室，为化学系，药物化学系，药物化学系，药理学和毒理学，KU医学中心和生物医学和生物分析研究。主要需求是增加快速原子轰击（FAB）能力。这已经是一个既定的服务实验室的能力和样品量不断增长，10年。一目前共有30个小组使用该实验室，本财政年度，实验室已经运行了1690个样本，到2015年将轻松达到2500个样本。七月这项工作是在三个设施仪器上进行的， Varian-NUT CH 5，Nermag四极杆GC/MS系统和高分辨率 VG ZAB HS. Nermag和ZAB用于大部分样品。 CH 5（1970年购买）作为FAB仪器已经过时。 Nermag是目前在探针和GC实验中使用饱和。 ZAB已经用常规精确质量和FAB分析达到接近饱和。从1989年7月开始，对FAB分析的需求增加了6 时间从去年。预计FAB的工作量将再次翻一番，进行肽分子量实验以支持四组才刚刚开始使用质谱分析法来鉴定多肽。肽项目涉及识别和定位小肽中的修饰氨基酸。的MS/MS能力杂交体将用于提供序列信息， FAB实验单位质量前体和碎片离子分辨率在CAD实验中用杂交获得的肽将是至关重要的涉及溴和氘标记肽的项目。两组目前正在合成配位化合物，并已成为主要的 FAB用户确认中间体和最终产品。两研究小组在从它们的分子中获得分子离子方面取得了好坏参半的成功，含金属的样品。经常观察到脱金属物质在FAB光谱中。类似的化合物已被菲尔德解吸（FD）。 FD用于配位化合物提供了一种新的方法。软电离方法，避免了矩阵化学的复杂性。