HYBRID (EBQQ) MASS SPECTROMETER

混合型 (EBQQ) 质谱仪

基本信息

批准号：
3520993
负责人：
TODD D WILLIAMS
金额：
$ 38.5万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-06-20 至 1992-06-19
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3520993
关键词：
biomedical equipment purchase biomedical equipment resource mass spectrometry

项目摘要

This request is for a Hybrid Mass Spectrometer to be part of the Mass Spectrometry Laboratory at the University of Kansas, serving the Departments of Chemistry, Medicinal Chemistry, Pharmaceutical Chemistry, Pharmacology and Toxicology, KU Medical Center and the centers for Biomedical and Bioanalytical Research. The primary need is for increased fast atom bombardment (FAB) capability. This has been an established service lab with growing capability and sample load for 10 years. A total of thirty groups currently use the lab and at 7/12 through the fiscal year the lab has run 1690 samples and will easily reach 2500 by July. This work is being carried out on three facility instruments,a Varian-NUT CH5, a Nermag quadrupole GC/MS system, and a high resolution VG ZAB HS. The Nermag and the ZAB are used for the bulk of the samples. The CH5 (bought in 1970) is obsolete as a FAB instrument. The Nermag is currently saturated in use by probe and GC experiments. The ZAB has reached near saturation with routine exact mass and FAB analysis. Starting in July of 1989 the demand for FAB analysis has increased 6 times from the previous year. FAB work is expected to double again as peptide molecular weight experiments are done in support of four groups just beginning to use mass spectrometry for peptide characterization. The peptide projects involve the identification and localization of modified amino acids in small peptides. The MS/MS capability of the hybrid will be used to provide the sequence information not available in the FAB experiments. The unit mass precursor and fragment ion resolution obtainable with a hybrid in CAD experiments will be vital for peptide projects involving bromine and deuterium labeled peptides. Two groups are currently synthesizing coordination compounds and have been the major FAB users for confirmation of intermediates and final products. Both groups have had mixed success in obtaining molecular ions from their metal containing samples. Frequently demetallated species are observed in the FAB spectra. Similar compounds have been characterized by Field Desroption (FD). The use of FD for coordination compounds provides a soft ionization method that avoids the complications of matrix chemistry.

该要求是混合质谱仪成为质量的一部分堪萨斯大学的光谱实验室，为化学部门，药物化学，药物化学，药理学和毒理学，KU医疗中心和中心生物医学和生物分析研究。主要需求是增加快速原子轰击（FAB）功能。这是一个已建立的服务实验室具有增长能力和样品负载10年。一个目前总共有30个小组使用该实验室，并在7/12通过财政年度该实验室已运行1690个样本，可轻松达到2500 七月。这项工作是在三种设施仪器上进行的 Varian-Nut CH5，Nerag Quadrupole GC/MS系统和高分辨率 VG ZAB HS。 Nermag和Zab用于大部分样品。 CH5（1970年购买）被过时用作工具仪器。 Nermag是目前通过探针和GC实验饱和。 Zab有通过常规精确质量和FAB分析，达到饱和度接近饱和。从1989年7月开始，对晶圆厂分析的需求增加了6 从上一年开始的时间。预计工厂工作将再次加倍肽分子量实验是为了支持四组的刚刚开始使用质谱法进行肽表征。肽项目涉及识别和本地化小肽中的修饰氨基酸。 MS/MS功能混合动力将用于提供序列信息 FAB实验。单位质量前体和碎片离子分辨率 CAD实验中的混合动力车可获得对于肽至关重要涉及溴和氘标记肽的项目。两组目前正在合成协调化合物，并且已经是主要的用于确认中间体和最终产品的Fab用户。两个都小组在从其分子离子中获得了混合成功的成功包含样品的金属。经常观察到被灭绝的物种在Fab Spectra中。类似化合物的特征是字段 Desroption（FD）。使用FD进行协调化合物提供了软电离法避免了基质化学的并发症。